First published online July 18, 2002; 10.1104/pp.003327
Plant Physiol, August 2002, Vol. 129, pp. 1544-1556
Activation Tagging Using the En-I Maize Transposon
System in Arabidopsis
Nayelli
Marsch-Martinez,
Raffaella
Greco,
Gert
Van Arkel,
Luis
Herrera-Estrella, and
Andy
Pereira*
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The
Netherlands (N.M.-M., R.G., G.V.A., A.P.); and Centro de
Investigación y de Estudios Avanzados-Instituto Politécnico
Nacional-Irapuato, P.O. Box 629, 3500 Irapuato, Guanajuato,
México (N.M.-M., L.H.-E.)
A method for the generation of stable activation tag inserts
was developed in Arabidopsis using the maize (Zea mays)
En-I transposon system. The method employs
greenhouse selectable marker genes that are useful to efficiently
generate large populations of insertions. A population of about
8,300 independent stable activation tag inserts has been produced.
Greenhouse-based screens for mutants in a group of plants containing
about 2,900 insertions revealed about 31 dominant mutants, suggesting a
dominant mutant frequency of about 1%. From the first batch of about
400 stable insertions screened in the greenhouse, four
gain-in-function, dominant activation-tagged, morphological mutants
were identified. A novel gain-in-function mutant called
thread is described, in which the target gene belongs to
the same family as the YUCCA flavin-mono-oxygenase that was identified
by T-DNA activation tagging. The high frequency of identified
gain-in-function mutants in the population suggests that the
En-I system described here is an efficient strategy to
saturate plant genomes with activation tag inserts. Because only a
small number of primary transformants are required to generate an
activation tag population, the En-I system appears to be
an attractive alternative to study plant species where the present
transformation methods have low efficiencies.
*
Corresponding author; e-mail A.Pereira{at}plant.wag-ur.nl; fax
31-317-418094.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
T. Kuromori, S. Takahashi, Y. Kondou, K. Shinozaki, and M. Matsui
Phenome Analysis in Plant Species Using Loss-of-Function and Gain-of-Function Mutants
Plant Cell Physiol.,
July 1, 2009;
50(7):
1215 - 1231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Trigueros, M. Navarrete-Gomez, S. Sato, S. K. Christensen, S. Pelaz, D. Weigel, M. F. Yanofsky, and C. Ferrandiz
The NGATHA Genes Direct Style Development in the Arabidopsis Gynoecium
PLANT CELL,
May 1, 2009;
21(5):
1394 - 1409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Qu, A. Desai, R. Wing, and V. Sundaresan
A Versatile Transposon-Based Activation Tag Vector System for Functional Genomics in Cereals and Other Monocot Plants
Plant Physiology,
January 1, 2008;
146(1):
189 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. I. Kim, A. Sharkhuu, J. B. Jin, P. Li, J. C. Jeong, D. Baek, S. Y. Lee, J. J. Blakeslee, A. S. Murphy, H. J. Bohnert, et al.
yucca6, a Dominant Mutation in Arabidopsis, Affects Auxin Accumulation and Auxin-Related Phenotypes
Plant Physiology,
November 1, 2007;
145(3):
722 - 735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Karaba, S. Dixit, R. Greco, A. Aharoni, K. R. Trijatmiko, N. Marsch-Martinez, A. Krishnan, K. N. Nataraja, M. Udayakumar, and A. Pereira
Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene
PNAS,
September 25, 2007;
104(39):
15270 - 15275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Ahn, J. Kim, S. J. Yoo, S. Y. Yoo, H. Roh, J.-H. Choi, M. S. Choi, K. S. Chung, E. J. Han, S. M. Hong, et al.
Isolation of 151 Mutants that Have Developmental Defects from T-DNA Tagging
Plant Cell Physiol.,
January 1, 2007;
48(1):
169 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Cheng, X. Dai, and Y. Zhao
Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis.
Genes & Dev.,
July 1, 2006;
20(13):
1790 - 1799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Woodward, S. M. Bemis, E. J. Hill, S. Sawa, T. Koshiba, and K. U. Torii
Interaction of Auxin and ERECTA in Elaborating Arabidopsis Inflorescence Architecture Revealed by the Activation Tagging of a New Member of the YUCCA Family Putative Flavin Monooxygenases
Plant Physiology,
September 1, 2005;
139(1):
192 - 203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aharoni, S. Dixit, R. Jetter, E. Thoenes, G. van Arkel, and A. Pereira
The SHINE Clade of AP2 Domain Transcription Factors Activates Wax Biosynthesis, Alters Cuticle Properties, and Confers Drought Tolerance when Overexpressed in Arabidopsis
PLANT CELL,
September 1, 2004;
16(9):
2463 - 2480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aharoni, A. P. Giri, S. Deuerlein, F. Griepink, W.-J. de Kogel, F. W. A. Verstappen, H. A. Verhoeven, M. A. Jongsma, W. Schwab, and H. J. Bouwmeester
Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants
PLANT CELL,
December 1, 2003;
15(12):
2866 - 2884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Parenicova, S. de Folter, M. Kieffer, D. S. Horner, C. Favalli, J. Busscher, H. E. Cook, R. M. Ingram, M. M. Kater, B. Davies, et al.
Molecular and Phylogenetic Analyses of the Complete MADS-Box Transcription Factor Family in Arabidopsis: New Openings to the MADS World
PLANT CELL,
July 1, 2003;
15(7):
1538 - 1551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Pan, H. Liu, J. Clarke, J. Jones, M. Bevan, and L. Stein
ATIDB: Arabidopsis thaliana insertion database
Nucleic Acids Res.,
February 15, 2003;
31(4):
1245 - 1251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-H. Jeong, S. An, H.-G. Kang, S. Moon, J.-J. Han, S. Park, H. S. Lee, K. An, and G. An
T-DNA Insertional Mutagenesis for Activation Tagging in Rice
Plant Physiology,
December 1, 2002;
130(4):
1636 - 1644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
