First published online July 25, 2002; 10.1104/pp.003707
Plant Physiol, August 2002, Vol. 129, pp. 1568-1580
Significance of the Expression of the CER6 Condensing Enzyme for
Cuticular Wax Production in Arabidopsis1
Tanya S.
Hooker,
Anthony A.
Millar,2 and
Ljerka
Kunst*
Department of Botany, University of British Columbia, Vancouver,
British Columbia V6T 1Z4, Canada
To learn more about the role of the CER6 condensing enzyme
in Arabidopsis surface wax production, we determined
CER6 transcription domains and the timing of
CER6 transcription in vegetative and reproductive
structures from juvenile, mature, and senescing tissues. We found that
CER6 is highly transcribed throughout development, exclusively in the epidermal cells in all tissues examined. The only
exception to the epidermal expression was observed in anthers nearing
maturity, in which CER6 mRNA was localized in the
tapetum. To determine if environmental factors such as light and water deficit, which are known to stimulate wax accumulation, induce CER6 transcription, we examined the effects of these
factors on CER6 transcript abundance. Our results
demonstrate that light is essential for CER6
transcription, and that osmotic stress and the presence of abscisic
acid enhance CER6 transcript accumulation. CER6 promoter-directed expression of the
 -glucuronidase reporter gene in transgenic plants
demonstrated that the CER6 promoter was highly effective in
directing epidermis-specific expression in Arabidopsis and tobacco
(Nicotiana tabacum). Furthermore, CER6 promoter-driven CER6 overexpression resulted in
increased wax deposition in Arabidopsis stems. These experiments
indicate that the expression level of CER6 in the
epidermis is one of the factors controlling wax accumulation on
Arabidopsis stems.
1
This work was supported by the Natural Sciences
and Engineering Research Council of Canada (grant to L.K.).
2
Present address: Commonwealth Scientific and Industrial
Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia.
*
Corresponding author; e-mail kunst{at}interchange.ubc.ca; fax
604-822-6089.
© 2002 American Society of Plant Physiologists
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