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First published online July 25, 2002; 10.1104/pp.003707

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Plant Physiol, August 2002, Vol. 129, pp. 1568-1580

Significance of the Expression of the CER6 Condensing Enzyme for Cuticular Wax Production in Arabidopsis1

Tanya S. Hooker, Anthony A. Millar,2 and Ljerka Kunst*

Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada

To learn more about the role of the CER6 condensing enzyme in Arabidopsis surface wax production, we determined CER6 transcription domains and the timing of CER6 transcription in vegetative and reproductive structures from juvenile, mature, and senescing tissues. We found that CER6 is highly transcribed throughout development, exclusively in the epidermal cells in all tissues examined. The only exception to the epidermal expression was observed in anthers nearing maturity, in which CER6 mRNA was localized in the tapetum. To determine if environmental factors such as light and water deficit, which are known to stimulate wax accumulation, induce CER6 transcription, we examined the effects of these factors on CER6 transcript abundance. Our results demonstrate that light is essential for CER6 transcription, and that osmotic stress and the presence of abscisic acid enhance CER6 transcript accumulation. CER6 promoter-directed expression of the beta -glucuronidase reporter gene in transgenic plants demonstrated that the CER6 promoter was highly effective in directing epidermis-specific expression in Arabidopsis and tobacco (Nicotiana tabacum). Furthermore, CER6 promoter-driven CER6 overexpression resulted in increased wax deposition in Arabidopsis stems. These experiments indicate that the expression level of CER6 in the epidermis is one of the factors controlling wax accumulation on Arabidopsis stems.

1 This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant to L.K.).

2 Present address: Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia.

* Corresponding author; e-mail kunst{at}interchange.ubc.ca; fax 604-822-6089.

© 2002 American Society of Plant Physiologists


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