Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online June 28, 2002; 10.1104/pp.011025

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
129/4/1755    most recent
pp.011025v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (52)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, D. L.
Right arrow Articles by Gross, K. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, D. L.
Right arrow Articles by Gross, K. C.
Agricola
Right arrow Articles by Smith, D. L.
Right arrow Articles by Gross, K. C.

Plant Physiol, August 2002, Vol. 129, pp. 1755-1762

Down-Regulation of Tomato beta -Galactosidase 4 Results in Decreased Fruit Softening1

David L. Smith, Judith A. Abbott, and Kenneth C. Gross*

Produce Quality and Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 002, 10300 Baltimore Avenue, Beltsville, Maryland 20705-2350

Transcript abundance of the gene encoding beta -galactosidase II, a beta -galactosidase/exo-galactanase (EC 3.2.1.23) present during tomato (Lycopersicon esculentum) fruit ripening, was suppressed by expression of an antisense tomato beta -galactosidase 4 (TBG4) cDNA driven by the cauliflower mosaic virus 35S promoter. RNA gel-blot analysis was used to evaluate TBG4 mRNA levels in transgenic fruit. All of the antisense lines had attenuated TBG4 mRNA levels in turning stage fruit; however, TBG4 mRNA suppression was unstable, and mRNA levels varied in red-ripe fruit among the lines. Suppression of TBG4 mRNA levels in antisense fruit was correlated with a reduction in extractable exo-galactanase activity against a lupin galactan. All of the antisense lines had reduced free galactose levels at mature green stage 4, but levels comparable with controls during ripening. Total cell wall galactosyl contents in the antisense fruit were not significantly different from control fruit. Whole-fruit firmness was measured using a texture analyzer and the means of the peak force measurements for four of six antisense lines were significantly higher than control fruit. One antisense line had red-ripe fruit that were 40% firmer than controls. Fruit from this antisense line also had the lowest TBG4 mRNA and exo-galactanase levels and the highest wall galactosyl content during the early stages of ripening, implicating an involvement of this gene product in cell wall modification leading to fruit softening.

1 Use of a company or product name by the U.S. Department of Agriculture does not imply approval or recommendation of the product to the exclusion of others which may also be suitable.

* Corresponding author; e-mail grossk{at}ba.ars.usda.gov; fax 301-504-5107.

© 2002 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Lycett
The role of Rab GTPases in cell wall metabolism
J. Exp. Bot., November 1, 2008; 59(15): 4061 - 4074.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Guillon, S. Philippe, B. Bouchet, M.-F. Devaux, P. Frasse, B. Jones, M. Bouzayen, and M. Lahaye
Down-regulation of an Auxin Response Factor in the tomato induces modification of fine pectin structure and tissue architecture
J. Exp. Bot., February 10, 2008; (2008) erm323v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Macquet, M.-C. Ralet, O. Loudet, J. Kronenberger, G. Mouille, A. Marion-Poll, and H. M. North
A Naturally Occurring Mutation in an Arabidopsis Accession Affects a {beta}-D-Galactosidase That Increases the Hydrophilic Potential of Rhamnogalacturonan I in Seed Mucilage
PLANT CELL, December 1, 2007; 19(12): 3990 - 4006.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Nishiyama, M. Guis, J. K. C. Rose, Y. Kubo, K. A. Bennett, L. Wangjin, K. Kato, K. Ushijima, R. Nakano, A. Inaba, et al.
Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon
J. Exp. Bot., April 1, 2007; 58(6): 1281 - 1290.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Fonseca, L. Monteiro, M. G. Barreiro, and M. S. Pais
Expression of genes encoding cell wall modifying enzymes is induced by cold storage and reflects changes in pear fruit texture
J. Exp. Bot., August 1, 2005; 56(418): 2029 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T.-W. Wang, C.-G. Zhang, W. Wu, L. M. Nowack, E. Madey, and J. E. Thompson
Antisense Suppression of Deoxyhypusine Synthase in Tomato Delays Fruit Softening and Alters Growth and Development
Plant Physiology, July 1, 2005; 138(3): 1372 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Tateishi, H. Mori, J. Watari, K. Nagashima, S. Yamaki, and H. Inoue
Isolation, Characterization, and Cloning of {alpha}-L-Arabinofuranosidase Expressed during Fruit Ripening of Japanese Pear
Plant Physiology, July 1, 2005; 138(3): 1653 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, S. Thoiron, and M. Lahaye
Physico-chemical characteristics of cell walls from Arabidopsis thaliana microcalli showing different adhesion strengths
J. Exp. Bot., September 1, 2004; 55(405): 2087 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. A. Brummell, V. Dal Cin, C. H. Crisosto, and J. M. Labavitch
Cell wall metabolism during maturation, ripening and senescence of peach fruit
J. Exp. Bot., September 1, 2004; 55(405): 2029 - 2039.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Wu and J. K. Burns
A {beta}-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis)
J. Exp. Bot., July 1, 2004; 55(402): 1483 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. J. Giovannoni
Genetic Regulation of Fruit Development and Ripening
PLANT CELL, June 1, 2004; 16(suppl_1): S170 - S180.
[Full Text] [PDF]

Home page
 J Exp BotHome page
E. Moctezuma, D. L. Smith, and K. C. Gross
Antisense suppression of a {beta}-galactosidase gene (TB G6) in tomato increases fruit cracking
J. Exp. Bot., September 1, 2003; 54(390): 2025 - 2033.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists