First published online July 11, 2002; 10.1104/pp.002618
Plant Physiol, August 2002, Vol. 129, pp. 1788-1794
Identification of a Soybean Protein That Interacts with GAGA
Element Dinucleotide Repeat DNA1
Indu
Sangwan and
Mark R.
O'Brian*
Department of Biochemistry, State University of New York, Buffalo,
New York 14214
Dinucleotide repeat DNA with the pattern
(GA)n/(TC)n, so-called GAGA elements, control
gene expression in animals, and are recognized by a specific regulatory
protein. Here, a yeast one-hybrid screen was used to isolate
soybean (Glycine max) cDNA encoding a GAGA-binding
protein (GBP) that binds to (GA)n/(CT)n
DNA. Soybean GBP was dissimilar from the GAGA factor of
Drosophila melanogaster. Recombinant GBP protein did not
bind to dinucleotide repeat sequences other than
(GA)n/(CT)n. GBP bound to the promoter of the
heme and chlorophyll synthesis gene Gsa1, which
contains a GAGA element. Removal of that GAGA element abrogated binding
of GBP to the promoter. Furthermore, insertion of the GAGA element to a
nonspecific DNA conferred GBP-binding activity on that DNA. Thus, the
GAGA element of the Gsa1 promoter is both necessary and
sufficient for GBP binding. Gbp mRNA was expressed in
leaves and was induced in symbiotic root nodules elicited by the
bacterium Bradyrhizobium japonicum. In addition,
Gbp transcripts were much higher in leaves of
dark-treated etiolated plantlets than in those exposed to light for
24 h. Homologs of GBP were found in other dicots and in the
monocot rice (Oryza sativa), as well. We suggest that
interaction between GAGA elements and GBP-like proteins is a regulatory
feature in plants.
1
This work was supported by the National Science
Foundation (grant no. MCB-0089928).
*
Corresponding author; e-mail mrobrian{at}buffalo.edu; fax
716-829-2725.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. K. Udvardi, K. Kakar, M. Wandrey, O. Montanari, J. Murray, A. Andriankaja, J.-Y. Zhang, V. Benedito, J. M.I. Hofer, F. Chueng, et al.
Legume Transcription Factors: Global Regulators of Plant Development and Response to the Environment
Plant Physiology,
June 1, 2007;
144(2):
538 - 549.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Casas-Mollano, N. T. Lao, and T. A. Kavanagh
Intron-regulated expression of SUVH3, an Arabidopsis Su(var)3-9 homologue
J. Exp. Bot.,
September 1, 2006;
57(12):
3301 - 3311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Newcomb, R. N. Crowhurst, A. P. Gleave, E. H.A. Rikkerink, A. C. Allan, L. L. Beuning, J. H. Bowen, E. Gera, K. R. Jamieson, B. J. Janssen, et al.
Analyses of Expressed Sequence Tags from Apple
Plant Physiology,
May 1, 2006;
141(1):
147 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-L. Xiao, S. R. Smith, N. Ishmael, J. C. Redman, N. Kumar, E. L. Monaghan, M. Ayele, B. J. Haas, H. C. Wu, and C. D. Town
Analysis of the cDNAs of Hypothetical Genes on Arabidopsis Chromosome 2 Reveals Numerous Transcript Variants
Plant Physiology,
November 1, 2005;
139(3):
1323 - 1337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kooiker, C. A. Airoldi, A. Losa, P. S. Manzotti, L. Finzi, M. M. Kater, and L. Colombo
BASIC PENTACYSTEINE1, a GA Binding Protein That Induces Conformational Changes in the Regulatory Region of the Homeotic Arabidopsis Gene SEEDSTICK
PLANT CELL,
March 1, 2005;
17(3):
722 - 729.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pauli, H. M. Rothnie, G. Chen, X. He, and T. Hohn
The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region
J. Virol.,
November 15, 2004;
78(22):
12120 - 12128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
