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Plant Physiol, August 2002, Vol. 129, pp. 1899-1907
O-Methyltransferases Involved in the Biosynthesis of
Volatile Phenolic Derivatives in Rose Petals1
Noa
Lavid,
Jihong
Wang,
Moshe
Shalit,
Inna
Guterman,
Einat
Bar,
Till
Beuerle,
Naama
Menda,
Sharoni
Shafir,
Dani
Zamir,
Zach
Adam,
Alexander
Vainstein,
David
Weiss,
Eran
Pichersky, and
Efraim
Lewinsohn*
Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research
Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel (N.L., M.S.,
E.B., E.L.); Department of Molecular, Cellular, and Developmental
Biology, University of Michigan, Ann Arbor, Michigan 48109-1048 (J.W.,
T.B., E.P.); and Faculty of Agricultural, Food, and Environmental
Quality Science, The Hebrew University of Jerusalem, Rehovot, Israel
(I.G., N.M., S.S., D.Z., Z.A., A.V., D.W.)
Rose (Rosa hybrida) flowers produce and emit
a diverse array of volatiles, characteristic to their unique scent. One
of the most prominent compounds in the floral volatiles of many
rose varieties is the methoxylated phenolic derivative
3,5-dimethoxytoluene (orcinol dimethyl ether). Cell-free extracts
derived from developing rose petals displayed
O-methyltransferase (OMT) activities toward several
phenolic substrates, including 3,5-dihydroxytoluene (orcinol), 3-methoxy,5-hydroxytoluene (orcinol monomethyl ether), 1-methoxy, 2-hydroxy benezene (guaiacol), and eugenol. The activity was most prominent in rose cv Golden Gate, a variety that produces relatively high levels of orcinol dimethyl ether, as compared with rose cv Fragrant Cloud, an otherwise scented variety but which emits almost no
orcinol dimethyl ether. Using a functional genomics approach, we have
identified and characterized two closely related cDNAs from a rose
petal library that each encode a protein capable of methylating the
penultimate and immediate precursors (orcinol and orcinol monomethyl
ether, respectively) to give the final orcinol dimethyl ether product.
The enzymes, designated orcinol OMTs (OOMT1 and OOMT2), are closely
related to other plant methyltransferases whose substrates range from
isoflavones to phenylpropenes. The peak in the levels of
OOMT1 and OOMT2 transcripts in the
flowers coincides with peak OMT activity and with the emission of
orcinol dimethyl ether.
1
This work was supported by the Israeli Ministry
of Science, Culture and Sports (grant no. 1410-2-00 to E.L., D.Z.,
Z.A., A.V., and D.W.), by a Binational Agricultural Research and
Development Fund scholarship (to E.P.), by the National Science
Foundation (grant no. MCB-9974463 to E.P.), by the United
States-Israel Binational Science Foundation and the Israel Science
Foundation (to S.S.), and by a Deutscher Akademischer Austauschdienst
fellowship (Gemeinsames Hochschulprogramm III von Bund und
Ländern; to T.B.). This is publication no. 113/2002 of the
Agricultural Research Organization (Bet Dagan, Israel).
*
Corresponding author; e-mail twefraim{at}volcani.agri.gov.il; fax
972-4-983-6936.
© 2002 American Society of Plant Physiologists
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