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Plant Physiol, August 2002, Vol. 129, pp. 1899-1907

O-Methyltransferases Involved in the Biosynthesis of Volatile Phenolic Derivatives in Rose Petals1

Noa Lavid, Jihong Wang, Moshe Shalit, Inna Guterman, Einat Bar, Till Beuerle, Naama Menda, Sharoni Shafir, Dani Zamir, Zach Adam, Alexander Vainstein, David Weiss, Eran Pichersky, and Efraim Lewinsohn*

Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, 30095, Israel (N.L., M.S., E.B., E.L.); Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048 (J.W., T.B., E.P.); and Faculty of Agricultural, Food, and Environmental Quality Science, The Hebrew University of Jerusalem, Rehovot, Israel (I.G., N.M., S.S., D.Z., Z.A., A.V., D.W.)

Rose (Rosa hybrida) flowers produce and emit a diverse array of volatiles, characteristic to their unique scent. One of the most prominent compounds in the floral volatiles of many rose varieties is the methoxylated phenolic derivative 3,5-dimethoxytoluene (orcinol dimethyl ether). Cell-free extracts derived from developing rose petals displayed O-methyltransferase (OMT) activities toward several phenolic substrates, including 3,5-dihydroxytoluene (orcinol), 3-methoxy,5-hydroxytoluene (orcinol monomethyl ether), 1-methoxy, 2-hydroxy benezene (guaiacol), and eugenol. The activity was most prominent in rose cv Golden Gate, a variety that produces relatively high levels of orcinol dimethyl ether, as compared with rose cv Fragrant Cloud, an otherwise scented variety but which emits almost no orcinol dimethyl ether. Using a functional genomics approach, we have identified and characterized two closely related cDNAs from a rose petal library that each encode a protein capable of methylating the penultimate and immediate precursors (orcinol and orcinol monomethyl ether, respectively) to give the final orcinol dimethyl ether product. The enzymes, designated orcinol OMTs (OOMT1 and OOMT2), are closely related to other plant methyltransferases whose substrates range from isoflavones to phenylpropenes. The peak in the levels of OOMT1 and OOMT2 transcripts in the flowers coincides with peak OMT activity and with the emission of orcinol dimethyl ether.

1 This work was supported by the Israeli Ministry of Science, Culture and Sports (grant no. 1410-2-00 to E.L., D.Z., Z.A., A.V., and D.W.), by a Binational Agricultural Research and Development Fund scholarship (to E.P.), by the National Science Foundation (grant no. MCB-9974463 to E.P.), by the United States-Israel Binational Science Foundation and the Israel Science Foundation (to S.S.), and by a Deutscher Akademischer Austauschdienst fellowship (Gemeinsames Hochschulprogramm III von Bund und Ländern; to T.B.). This is publication no. 113/2002 of the Agricultural Research Organization (Bet Dagan, Israel).

* Corresponding author; e-mail twefraim{at}volcani.agri.gov.il; fax 972-4-983-6936.

© 2002 American Society of Plant Physiologists


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