First published online July 25, 2002; 10.1104/pp.001636
Plant Physiol, August 2002, Vol. 129, pp. 1921-1928
In Vivo Interactions between Photosynthesis, Mitorespiration, and
Chlororespiration in Chlamydomonas reinhardtii
Laurent
Cournac,*
Gwendal
Latouche,
Zoran
Cerovic,
Kevin
Redding,
Jacques
Ravenel, and
Gilles
Peltier
Commissariat à l'Energie Atomique (CEA) Cadarache,
Direction des Sciences du Vivant, Département
d'Ecophysiologie Végétale et de Microbiologie, Laboratoire
d'Ecophysiologie de la Photosynthèse, Unité Mixte de
Recherche 163 Centre National de la Recherche Scientifique CEA,
Univ-Méditerranée CEA 1000, F-13108
Saint-Paul-lez-Durance cedex, France (L.C., J.R., G.P.); Laboratoire
pour l'Utilisation du Rayonnement Electromagnétique-Centre
National de la Recherche Scientifique, Bat 203, Boîte Postale 34, Centre Universitaire Paris-Sud, Equipe Photosynthèse et
Télédétection, F-91898 Orsay, France (G.L., Z.C.);
and Department of Chemistry, 120 Lloyd Hall, 6th Avenue, University of
Alabama, Tuscaloosa, Alabama 35487-0336 (K.R.)
Interactions between photosynthesis, mitochondrial
respiration (mitorespiration), and chlororespiration have been
investigated in the green alga Chlamydomonas reinhardtii
using flash illumination and a bare platinum electrode. Depending on
the physiological status of algae, flash illumination was found to
induce either a fast (t1/2  300 ms) or slow
(t1/2 3 s) transient inhibition of oxygen
uptake. Based on the effects of the mitorespiratory inhibitors
myxothiazol and salicyl hydroxamic acid (SHAM), and of propyl gallate,
an inhibitor of the chlororespiratory oxidase, we conclude that the
fast transient is due to the flash-induced inhibition of
chlororespiration and that the slow transient is due to the
flash-induced inhibition of mitorespiration. By measuring blue-green
fluorescence changes, related to the redox status of the pyridine
nucleotide pool, and chlorophyll fluorescence, related to the redox
status of plastoquinones (PQs) in C.
reinhardtii wild type and in a photosystem I-deficient
mutant, we show that interactions between photosynthesis and
chlororespiration are favored when PQ and pyridine nucleotide pools are
reduced, whereas interactions between photosynthesis and
mitorespiration are favored at more oxidized states. We conclude that
the plastid oxidase, similar to the mitochondrial alternative oxidase,
becomes significantly engaged when the PQ pool becomes highly reduced,
and thereby prevents its over-reduction.
*
Corresponding author; e-mail laurent.cournac{at}cea.fr; fax
33-4-42-25-62-65.
© 2002 American Society of Plant Physiologists
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