First published online August 29, 2002; 10.1104/pp.003228
Plant Physiol, September 2002, Vol. 130, pp. 199-209
Indole Acetic Acid Distribution Coincides with Vascular
Differentiation Pattern during Arabidopsis Leaf
Ontogeny1
Orna
Avsian-Kretchmer,
Jin-Chen
Cheng,2
Lingjing
Chen,
Edgar
Moctezuma,3 and
Z. Renee
Sung*
Department of Plant and Microbial Biology, University of
California, 111 Koshland Hall, Berkeley, California 94720
We used an anti-indole acetic acid (IAA or auxin) monoclonal
antibody-based immunocytochemical procedure to monitor IAA level in
Arabidopsis tissues. Using immunocytochemistry and the IAA-driven  -glucuronidase (GUS) activity of Aux/IAA
promoter::GUS constructs to detect IAA distribution,
we investigated the role of polar auxin transport in vascular
differentiation during leaf development in Arabidopsis. We found that
shoot apical cells contain high levels of IAA and that IAA decreases as
leaf primordia expand. However, seedlings grown in the presence of IAA
transport inhibitors showed very low IAA signal in the shoot apical
meristem (SAM) and the youngest pair of leaf primordia. Older leaf
primordia accumulate IAA in the leaf tip in the presence or absence of
IAA transport inhibition. We propose that the IAA in the SAM and the youngest pair of leaf primordia is transported from outside sources, perhaps the cotyledons, which accumulate more IAA in the presence than
in the absence of transport inhibition. The temporal and spatial
pattern of IAA localization in the shoot apex indicates a change in IAA
source during leaf ontogeny that would influence flow direction and,
consequently, the direction of vascular differentiation. The IAA
production and transport pattern suggested by our results could explain
the venation pattern, and the vascular hypertrophy caused by IAA
transport inhibition. An outside IAA source for the SAM supports the
notion that IAA transport and procambium differentiation dictate
phyllotaxy and organogenesis.
1
This work was supported by the National Science
Foundation (grant no. DBI-9813361 to Z.R.S.).
2
Present address: College of Literature, Science,
and the Arts, University of Michigan, Ann Arbor, MI 48109.
3
Present address: U.S. Department of
Agriculture-Agricultural Research Service, Beltsville, MD 20705.
*
Corresponding author; e-mail zrsung{at}nature.berkeley.edu; fax
510-642-4995.
© 2002 American Society of Plant Physiologists
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