First published online August 16, 2002; 10.1104/pp.020007
Plant Physiol, September 2002, Vol. 130, pp. 221-233
Regulated Expression of Arabidopsis Phosphate
Transporters1
Athikkattuvalasu S.
Karthikeyan,
Deepa K.
Varadarajan,
Uthappa T.
Mukatira,
Matilde Paino
D'Urzo,
Barbara
Damsz, and
Kashchandra G.
Raghothama*
Department of Horticulture and Landscape Architecture, Purdue
University, West Lafayette, Indiana 47907-1165
Phosphorus deficiency is one of the major abiotic stresses
affecting plant growth. Plants respond to the persistent deficiency of
phosphate (Pi) by coordinating the expression of genes involved in
alleviation of the stress. The high-affinity Pi transporters are among
the major molecular determinants that are activated during Pi stress.
In this study, using three reporter genes (green fluorescent protein,
luciferase, and  -glucuronidase) regulated by two Pi transporter
promoters, we have carried out an extensive analysis of transcriptional
and spatial regulation of gene expression. Activation of the genes was
rapid, repressible, and specific in response to changes in Pi
availability. The phytohormones auxin and cytokinin suppressed the
expression of the reporter gene driven by the AtPT1
promoter, and that of the native gene, suggesting that hormones may be
involved in regulation of some component(s) of Pi starvation response
pathway. These studies also provide molecular evidence for a potential
role of high-affinity Pi transporters in mobilizing Pi into
reproductive organs. The results suggest that members of the Pi
transporter family may have similar but nonredundant functions in plants.
1
This work was supported by the U.S. Department
of Agriculture (grant no. 00-35100-9370 to K.G.R.). This is journal
paper no. 16,860 of the Purdue University Agricultural Research Program.
*
Corresponding author; e-mail ragu{at}hort.purdue.edu; fax
765-494-0391.
© 2002 American Society of Plant Physiologists
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