First published online August 8, 2002; 10.1104/pp.004168
Plant Physiol, September 2002, Vol. 130, pp. 265-272
Plasmalemma Abscisic Acid Perception Leads to RAB18
Expression via Phospholipase D Activation in Arabidopsis Suspension
Cells
Matthieu
Hallouin,
Thanos
Ghelis,1
Mathias
Brault,
Françoise
Bardat,
Daniel
Cornel,
Emile
Miginiac,
Jean-Pierre
Rona,
Bruno
Sotta, and
Emmanuelle
Jeannette*
Physiologie Cellulaire et Moléculaire des Plantes,
Unité Mixte de Recherche Centre National de la Recherche
Scientifique 7632, case 156, Université Pierre et Marie Curie
(Paris VI), 4 place Jussieu, 75252 Paris cedex 05, France (M.H., T.G.,
F.B., E.M., B.S., E.J.); and Electrophysiologie des Membranes, Equipe
Associée 3514, case 7069, Université Denis Diderot
(Paris 7), 2 place Jussieu, 75221 Paris cedex 05, France (M.B., D.C.,
J.-P.R.)
Abscisic acid (ABA) plays a key role in the control
of stomatal aperture by regulating ion channel activities and water
exchanges across the plasma membrane of guard cells. Changes in
cytoplasmic calcium content and activation of anion and
outward-rectifying K+ channels are among the earliest
cellular responses to ABA in guard cells. In Arabidopsis suspension
cells, we have demonstrated that outer plasmalemma perception of ABA
triggered similar early events. Furthermore, a Ca2+
influx and the activation of anion channels are part of the
ABA-signaling pathway leading to the specific expression of
RAB18. Here, we determine whether phospholipases are
involved in ABA-induced RAB18 expression. Phospholipase
C is not implicated in this ABA pathway. Using a transphosphatidylation
reaction, we show that ABA plasmalemma perception results in a
transient stimulation of phospholipase D (PLD) activity, which is
necessary for RAB18 expression. Further experiments
showed that PLD activation was unlikely to be regulated by
heterotrimeric G proteins. We also observed that ABA-dependent stimulation of PLD was necessary for the activation of plasma anion
current. However, when ABA activation of plasma anion channels was
inhibited, the ABA-dependent activation of PLD was unchanged. Thus, we
conclude that in Arabidopsis suspension cells, ABA stimulation of PLD
acts upstream from anion channels in the transduction pathway leading
to RAB18 expression.
1
Present address: University of Edinburgh,
Institute of Cell and Molecular Biology, Kings Buildings, Mayfield
Road, Edinburgh, EH9 3JH, Scotland, UK.
*
Corresponding author; e-mail ema{at}ccr.jussieu.fr; fax
33-1-44276232.
© 2002 American Society of Plant Physiologists
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