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Plant Physiol, September 2002, Vol. 130, pp. 312-324
Two New Loci, PLEIADE and HYADE,
Implicate Organ-Specific Regulation of Cytokinesis in
Arabidopsis1
Sabine
Müller,
Esther
Fuchs,2
Miroslav
Ovecka,
Joanna
Wysocka-Diller,3
Philip N.
Benfey, and
Marie-Theres
Hauser*
Center of Applied Genetics, University of Agricultural Sciences
Vienna, Muthgasse 18, A-1190 Vienna, Austria (S.M., E.F., M.-T.H.);
Slovak Academy of Sciences, Institute of Botany, SK-84223 Bratislava,
Slovak Republic (M.O.); and Department of Biology, New York University,
1009 Main Building, New York, New York 10003 (J.W.-D., P.N.B.)
In screens for regulators of root morphogenesis in
Arabidopsis we isolated six new recessive mutants with irregular cell
expansion. Complementation analyses placed the mutations in two
loci, PLEIADE (PLE) and
HYADE (HYA). Phenotypic analyses revealed
multinucleated cells, cell wall stubs, and synchronized cell divisions
in incompletely separated cells that are all characteristics of
defective cytokinesis. These defects were pronounced in roots and
undetectable in aerial organs. In addition, fertility and germination
were not affected by the mutations. Thus, the alleles that we have
isolated of PLE and HYA suggest that the
genes may encode organ-specific components needed primarily during root
development. Analysis of microtubule arrays during cell cycle in
ple and hya roots indicates that the presence of several synchronized nuclei influences the position of
preprophase band, mitotic spindles, and phragmoplasts. The enhanced and
synergistic phenotype of
PLE/ple.hya/hya
seedlings and double mutants point to a role of PLE and
HYA in the same process. These mutants provide tools to
elucidate the regulation of nuclear cytoskeletal interactions during
cell division and cytokinesis.
1
The initial stages of this work were
supported by the National Science Foundation (to P.N.B.) and by a
Schrödinger-Auslandsstipendium from the Austrian Science
Foundation (grant no. J0676-MOB to M.-T.H.). S.M. was supported by the
Austrian National Bank (Jubiläumsfondprojekt no. 5598) and by a
European Grant (no. PL-960217).
2
E.F. was an undergraduate student at the University of
Agricultural Sciences Vienna when she participated in this project.
3
Present address: Biological Sciences, Auburn University,
Auburn, AL 36849.
*
Corresponding author; e-mail hauser{at}mail.boku.ac.at; fax
43-1-36006-6392.
© 2002 American Society of Plant Physiologists
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