First published online August 8, 2002; 10.1104/pp.004622
Plant Physiol, September 2002, Vol. 130, pp. 325-333
Light-Intensity-Dependent Expression of Lhc Gene
Family Encoding Light-Harvesting Chlorophyll-a/b Proteins
of Photosystem II in Chlamydomonas
reinhardtii1
Haruhiko
Teramoto,
Akira
Nakamori,
Jun
Minagawa,2 and
Taka-aki
Ono*
Laboratory for Photobiology (1), Photodynamics Research Center, The
Institute of Physical and Chemical Research, Sendai 980-0845,
Japan
Excessive light conditions repressed the levels of mRNAs
accumulation of multiple Lhc genes encoding
light-harvesting chlorophyll-a/b (LHC) proteins of
photosystem (PS)II in the unicellular green alga, Chlamydomonas
reinhardtii. The light intensity required for the repression
tended to decrease with lowering temperature or CO2
concentration. The responses of six LhcII genes encoding the major LHC (LHCII) proteins and two genes (Lhcb4 and
Lhcb5) encoding the minor LHC proteins of PSII (CP29 and
CP26) were similar. The results indicate that the expression of these
Lhc genes is coordinately repressed when the energy
input through the antenna systems exceeds the requirement for
CO2 assimilation. The Lhc mRNA level
repressed under high-light conditions was partially recovered by adding
the electron transport inhibitor
3-(3,4-dichlorophenyl)-1,1-dimethylurea, suggesting that redox
signaling via photosynthetic electron carriers is involved in the gene
regulation. However, the mRNA level was still considerably lower under
high-light than under low-light conditions even in the presence of
3-(3,4-dichlorophenyl)-1,1-dimethylurea. Repression of the
Lhc genes by high light was prominent even in the
mutants deficient in the reaction center(s) of PSII or both PSI and
PSII. The results indicate that two alternative processes are involved
in the repression of Lhc genes under high-light
conditions, one of which is independent of the photosynthetic reaction
centers and electron transport events.
1
This research was supported by a grant for the
Frontier Research System at The Institute of Physical and Chemical
Research and by the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. 13640659).
2
Present address: The Institute of Low Temperature
Science, Hokkaido University, Sapporo 060-0819, Japan.
*
Corresponding author; e-mail takaaki{at}postman.riken.go.jp; fax
81-22-228-2045.
© 2002 American Society of Plant Physiologists
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