First published online August 16, 2002; 10.1104/pp.006163
Plant Physiol, September 2002, Vol. 130, pp. 415-421
Short-Term Boron Deprivation Inhibits Endocytosis of Cell Wall
Pectins in Meristematic Cells of Maize and Wheat Root
Apices1
Qin
Yu,2
Andrej
Hlavacka,
Toru
Matoh,
Dieter
Volkmann,
Diedrik
Menzel,
Heiner E.
Goldbach, and
Franti ek
Baluka*
Institute of Plant Nutrition, University of Bonn,
Karlrobert-Kreiten-Strasse 13, D-53115 Bonn, Germany (Q.Y., H.E.G.);
Laboratory of Plant Nutrition, Kyoto University, Kyoto 606-01, Japan
(T.M.); and Institute of Botany, Rheinische
Friedrich-Wilhelms-University of Bonn, Kirschallee 1, D-53115 Bonn,
Germany (A.H., D.V., D.M., F.B.)
By using immunofluorescence microscopy, we observed rapidly
altered distribution patterns of cell wall pectins in meristematic cells of maize (Zea mays) and wheat (Triticum
aestivum) root apices. This response was shown for
homogalacturonan pectins characterized by a low level (up to 40%) of
methylesterification and for rhamnogalacturonan II pectins cross-linked
by a borate diol diester. Under boron deprivation, abundance of these
pectins rapidly increased in cell walls, whereas their internalization
was inhibited, as evidenced by a reduced and even blocked accumulation
of these cell wall pectins within brefeldin A-induced compartments. In
contrast, root cells of species sensitive to the boron deprivation,
like zucchini (Cucurbita pepo) and alfalfa
(Medicago sativa), do not internalize cell wall pectins
into brefeldin A compartments and do not show accumulation of pectins
in their cell walls under boron deprivation. For maize and wheat root
apices, we favor an apoplastic target for the primary action of boron
deprivation, which signals deeper into the cell via
endocytosis-mediated pectin signaling along putative cell wall-plasma
membrane-cytoskeleton continuum.
1
This work was supported by the Deutsche
Forschungsgemeinschaft (grant no. Go 415/14-3, to H.E.G.), by the
Alexander von Humboldt Foundation (to Q.Y.), and by the Deutsches
Zentrum für Luft- und Raumfahrt (Bonn; to F.B. and D.V.). In
addition, F.B. receives partial support from the Slovak Academy of
Sciences, Grant Agency Vega (Bratislava, Slovakia; project no. 2031).
2
Present address: Faculty of Agriculture, WA Herbicide
Resistance Initiative, The University of Western Australia, Nedlands, WA 6907, Australia.
*
Corresponding author; e-mail baluska{at}uni-bonn.de; fax
49-228-739004.
© 2002 American Society of Plant Physiologists
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