Plant Physiol. PAM Fluorometers & Gas Exchange
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (78)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baluska, F.
Right arrow Articles by Volkmann, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baluska, F.
Right arrow Articles by Volkmann, D.
Agricola
Right arrow Articles by Baluska, F.
Right arrow Articles by Volkmann, D.

Plant Physiol, September 2002, Vol. 130, pp. 422-431

F-Actin-Dependent Endocytosis of Cell Wall Pectins in Meristematic Root Cells. Insights from Brefeldin A-Induced Compartments1

Frantisek Baluska,* Andrej Hlavacka, Jozef Samaj, Klaus Palme, David G. Robinson, Toru Matoh, David W. McCurdy, Diedrik Menzel, and Dieter Volkmann

Plant Cell Biology, Institute of Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany (F.B., A.H., D.M., D.V.); Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, SK-95007 Nitra, Slovakia (J.S.); Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, D-50829 Köln, Germany (K.P.); HIP Zellbiologie, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany (D.G.R.); Laboratory of Plant Nutrition, Kyoto University, Kyoto 606-01, Japan (T.M.); and School of Environmental and Life Sciences, The University of Newcastle, Newcastle, New South Wales 2308, Australia (D.W.M.)

Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but not all, cells. Here, we have analyzed assembly and distribution of BFA compartments in development- and tissue-specific contexts of growing maize (Zea mays) root apices. Surprisingly, these unique compartments formed only in meristematic cells of the root body. On the other hand, BFA compartments were absent from secretory cells of root cap periphery, metaxylem cells, and most elongating cells, all of which are active in exocytosis. We report that cell wall pectin epitopes counting rhamnogalacturonan II dimers cross-linked by borate diol diester, partially esterified (up to 40%) homogalacturonan pectins, and (1right-arrow4)-beta -D-galactan side chains of rhamnogalacturonan I were internalized into BFA compartments. In contrast, Golgi-derived secretory (esterified up to 80%) homogalacturonan pectins localized to the cytoplasm in control cells and did not accumulate within characteristic BFA compartments. Latrunculin B-mediated depolymerization of F-actin inhibited internalization and accumulation of cell wall pectins within intracellular BFA compartments. Importantly, cold treatment and protoplasting prevented internalization of wall pectins into root cells upon BFA treatment. These observations suggest that cell wall pectins of meristematic maize root cells undergo rapid endocytosis in an F-actin-dependent manner.

1 This work was supported by the Deutsches Zentrum für Luft- und Raumfahrt (Köln, Germany; to F.B. and D.V.) and by the Slovak Academy of Sciences, Grant Agency Vega (Bratislava, Slovakia; grant nos. 2031 and 2/616/99 to F.B. and J.S.).

* Correspondening author; e-mail baluska{at}uni-bonn.de; fax 49-228-739004.

© 2002 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J Exp BotHome page
E. Onelli, C. Prescianotto-Baschong, M. Caccianiga, and A. Moscatelli
Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold
J. Exp. Bot., August 1, 2008; 59(11): 3051 - 3068.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. G. Robinson, L. Jiang, and K. Schumacher
The Endosomal System of Plants: Charting New and Familiar Territories
Plant Physiology, August 1, 2008; 147(4): 1482 - 1492.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, and A. Geitmann
Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching
Plant Physiology, August 1, 2008; 147(4): 1646 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
D. Pozueta-Romero, P. Gonzalez, E. Etxeberria, and J. Pozueta-Romero
The Hyperbolic and Linear Phases of the Sucrose Accumulation Curve in Turnip Storage Cells Denote Carrier-mediated and Fluid Phase Endocytic Transport, Respectively
J. Amer. Soc. Hort. Sci., July 1, 2008; 133(4): 612 - 618.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Gonzalez-Aguero, L. Pavez, F. Ibanez, I. Pacheco, R. Campos-Vargas, L. A. Meisel, A. Orellana, J. Retamales, H. Silva, M. Gonzalez, et al.
Identification of woolliness response genes in peach fruit after post-harvest treatments
J. Exp. Bot., May 3, 2008; (2008) ern069v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. A. Collings, L. K. Gebbie, P. A. Howles, U. A. Hurley, R. J. Birch, A. H. Cork, C. H. Hocart, T. Arioli, and R. E. Williamson
Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion
J. Exp. Bot., February 5, 2008; (2008) erm324v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Siedlecka, S. Wiklund, M.-A. Peronne, F. Micheli, J. Lesniewska, I. Sethson, U. Edlund, L. Richard, B. Sundberg, and E. J. Mellerowicz
Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of Populus
Plant Physiology, February 1, 2008; 146(2): 554 - 565.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. T. Lee, C. Y. Y. Szeto, T. P. Ng, and H. S. Kwan
Endocytosis in the Shiitake Mushroom Lentinula edodes and Involvement of GTPase LeRAB7
Eukaryot. Cell, December 1, 2007; 6(12): 2406 - 2418.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Moscatelli, F. Ciampolini, S. Rodighiero, E. Onelli, M. Cresti, N. Santo, and A. Idilli
Distinct endocytic pathways identified in tobacco pollen tubes using charged nanogold
J. Cell Sci., November 1, 2007; 120(21): 3804 - 3819.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Muller, U. Mettbach, D. Menzel, and J. Samaj
Molecular Dissection of Endosomal Compartments in Plants
Plant Physiology, October 1, 2007; 145(2): 293 - 304.
[Full Text] [PDF]

Home page
 Am. J. Bot.Home page
E. Etxeberria, P. Gonzalez, and J. Pozueta-Romero
Mannitol-enhanced, fluid-phase endocytosis in storage parenchyma cells of celery (Apium graveolens; Apiaceae) petioles
Am. J. Botany, June 1, 2007; 94(6): 1041 - 1045.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. J. Haas, M. K. Sliwinski, D. E. Martinez, M. Preuss, K. Ebine, T. Ueda, E. Nielsen, G. Odorizzi, and M. S. Otegui
The Arabidopsis AAA ATPase SKD1 Is Involved in Multivesicular Endosome Function and Interacts with Its Positive Regulator LYST-INTERACTING PROTEIN5
PLANT CELL, April 1, 2007; 19(4): 1295 - 1312.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Chen, N. Teng, X. Wu, Y. Wang, W. Tang, J. Samaj, F. Baluska, and J. Lin
Disruption of Actin Filaments by Latrunculin B Affects Cell Wall Construction in Picea meyeri Pollen Tube by Disturbing Vesicle Trafficking
Plant Cell Physiol., January 1, 2007; 48(1): 19 - 30.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. K. Lam, C. L. Siu, S. Hillmer, S. Jang, G. An, D. G. Robinson, and L. Jiang
Rice SCAMP1 Defines Clathrin-Coated, trans-Golgi-Located Tubular-Vesicular Structures as an Early Endosome in Tobacco BY-2 Cells
PLANT CELL, January 1, 2007; 19(1): 296 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Tamura, H. Takahashi, T. Kunieda, K. Fuji, T. Shimada, and I. Hara-Nishimura
Arabidopsis KAM2/GRV2 Is Required for Proper Endosome Formation and Functions in Vacuolar Sorting and Determination of the Embryo Growth Axis
PLANT CELL, January 1, 2007; 19(1): 320 - 332.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Illes, M. Schlicht, J. Pavlovkin, I. Lichtscheidl, F. Baluska, and M. Ovecka
Aluminium toxicity in plants: internalization of aluminium into cells of the transition zone in Arabidopsis root apices related to changes in plasma membrane potential, endosomal behaviour, and nitric oxide production
J. Exp. Bot., December 1, 2006; 57(15): 4201 - 4213.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Pastuglia, J. Azimzadeh, M. Goussot, C. Camilleri, K. Belcram, J.-L. Evrard, A.-C. Schmit, P. Guerche, and D. Bouchez
{gamma}-Tubulin Is Essential for Microtubule Organization and Development in Arabidopsis
PLANT CELL, June 1, 2006; 18(6): 1412 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Boutte, M.-T. Crosnier, N. Carraro, J. Traas, and B. Satiat-Jeunemaitre
The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins
J. Cell Sci., April 1, 2006; 119(7): 1255 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Q. Wang, L. Kong, H. Hao, X. Wang, J. Lin, J. Samaj, and F. Baluska
Effects of Brefeldin A on Pollen Germination and Tube Growth. Antagonistic Effects on Endocytosis and Secretion
Plant Physiology, December 1, 2005; 139(4): 1692 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Zheng, O. Rowland, and L. Kunst
Disruptions of the Arabidopsis Enoyl-CoA Reductase Gene Reveal an Essential Role for Very-Long-Chain Fatty Acid Synthesis in Cell Expansion during Plant Morphogenesis
PLANT CELL, May 1, 2005; 17(5): 1467 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. K. Gebbie, J. E. Burn, C. H. Hocart, and R. E. Williamson
Genes encoding ADP-ribosylation factors in Arabidopsis thaliana L. Heyn.; genome analysis and antisense suppression
J. Exp. Bot., April 1, 2005; 56(414): 1079 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Bloch, M. Lavy, Y. Efrat, I. Efroni, K. Bracha-Drori, M. Abu-Abied, E. Sadot, and S. Yalovsky
Ectopic Expression of an Activated RAC in Arabidopsis Disrupts Membrane Cycling
Mol. Biol. Cell, April 1, 2005; 16(4): 1913 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Kim, M. Park, S. J. Kim, and I. Hwang
Actin Filaments Play a Critical Role in Vacuolar Trafficking at the Golgi Complex in Plant Cells
PLANT CELL, March 1, 2005; 17(3): 888 - 902.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Etxeberria, E. Baroja-Fernandez, F. J. Munoz, and J. Pozueta-Romero
Sucrose-inducible Endocytosis as a Mechanism for Nutrient Uptake in Heterotrophic Plant Cells
Plant Cell Physiol., March 1, 2005; 46(3): 474 - 481.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Russinova, J.-W. Borst, M. Kwaaitaal, A. Cano-Delgado, Y. Yin, J. Chory, and S. C. de Vries
Heterodimerization and Endocytosis of Arabidopsis Brassinosteroid Receptors BRI1 and AtSERK3 (BAK1)
PLANT CELL, December 1, 2004; 16(12): 3216 - 3229.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Samaj, F. Baluska, B. Voigt, M. Schlicht, D. Volkmann, and D. Menzel
Endocytosis, Actin Cytoskeleton, and Signaling
Plant Physiology, July 1, 2004; 135(3): 1150 - 1161.
[Full Text] [PDF]

Home page
 J Exp BotHome page
F. Baluska, J. Samaj, A. Hlavacka, J. Kendrick-Jones, and D. Volkmann
Actin-dependent fluid-phase endocytosis in inner cortex cells of maize root apices
J. Exp. Bot., February 1, 2004; 55(396): 463 - 473.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Boonsirichai, J. C. Sedbrook, R. Chen, S. Gilroy, and P. H. Masson
ALTERED RESPONSE TO GRAVITY Is a Peripheral Membrane Protein That Modulates Gravity-Induced Cytoplasmic Alkalinization and Lateral Auxin Transport in Plant Statocytes
PLANT CELL, November 1, 2003; 15(11): 2612 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Baluska, J. Samaj, P. Wojtaszek, D. Volkmann, and D. Menzel
Cytoskeleton-Plasma Membrane-Cell Wall Continuum in Plants. Emerging Links Revisited
Plant Physiology, October 1, 2003; 133(2): 482 - 491.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Nebenfuhr, C. Ritzenthaler, and D. G. Robinson
Brefeldin A: Deciphering an Enigmatic Inhibitor of Secretion
Plant Physiology, November 1, 2002; 130(3): 1102 - 1108.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
Q. Yu, A. Hlavacka, T. Matoh, D. Volkmann, D. Menzel, H. E. Goldbach, and F. Baluska
Short-Term Boron Deprivation Inhibits Endocytosis of Cell Wall Pectins in Meristematic Cells of Maize and Wheat Root Apices
Plant Physiology, September 1, 2002; 130(1): 415 - 421.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2002 by the American Society of Plant Biologists