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Plant Physiol, September 2002, Vol. 130, pp. 68-77
VFL, the Grapevine FLORICAULA/LEAFY
Ortholog, Is Expressed in Meristematic Regions Independently of Their
Fate1
María José
Carmona,2
Pilar
Cubas,2 and
José M.
Martínez-Zapater*
Departamento de Biotecnología, Escuela Técnica
Superior Ingenieros Agrónomos, Universidad Politécnica de
Madrid, Avenida Complutense s/n, 28040 Madrid, Spain (M.J.C.);
Departamento de Biotecnología, Instituto Nacional de
Investigación y Tecnología Agraria y Alimentaria,
Carretera de La Coruña km 7, 28040 Madrid, Spain (P.C.,
J.M.M.-Z.); and Departamento de Genética Molecular de Plantas,
Centro Nacional de Biotecnología, Consejo Superior de
Investigaciones Científicas, Campus de la Universidad
Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain (P.C.,
J.M.M.-Z.)
The flowering process in grapevine (Vitis
vinifera) takes place in buds and extends for two consecutive
growing seasons. To understand the genetic and molecular mechanisms
underlying this process, we have characterized grapevine bud
development, cloned the grapevine
FLORICAULA/LEAFY (FLO/LFY)
ortholog, VFL, and analyzed its expression patterns
during vegetative and reproductive development. Flowering induction
takes place during the first season. Upon induction, the shoot apical
meristem begins to produce lateral meristems that will give rise to
either inflorescences or tendrils. During the second season, after a
winter dormancy period, buds reactivate and inflorescence meristems
give rise to flower meristems. VFL is expressed in
lateral meristems that give rise to inflorescence and flower meristems,
consistent with a role in reproductive development. Furthermore,
VFL is also detected in other meristematic regions such
as the vegetative shoot apical meristem and the lateral meristems that
will give rise to tendrils. VFL is also expressed in
leaf primordia and in growing leaf margins until later stages of
development. Accumulation of VFL transcripts in
cell-proliferating regions suggests a role for VFL not
only in flower meristem specification, but also in the maintenance of
indeterminacy before the differentiation of derivatives of the apical
meristem: flowers, leaves, or tendrils.
1
This work was supported by the Comunidad
Autónoma de Madrid (grant no. 07B/0035/1999) and the Ministerio
de Educación Ciencia, Spain (postdoctoral fellowship to P.C.).
Support to research activity at Centro Nacional de
Biotecnología is provided through specific agreement of Consejo
Superior de Investigaciones Científicas-Instituto Nacional de
Investigacion y Tecnologia Agraria y Alimentaria.
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail zapater{at}cnb.uam.es; fax 34-915854506.
© 2002 American Society of Plant Physiologists
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