First published online September 20, 2002; 10.1104/pp.009175
Plant Physiol, October 2002, Vol. 130, pp. 577-590
Expression Profiling of the Whole Arabidopsis Shaggy-Like Kinase
Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain
Reaction1
Bénédicte
Charrier,*
Anthony
Champion,
Yves
Henry, and
Martin
Kreis
Laboratoire de Biologie du Développement des Plantes,
Institut de Biotechnologie des Plantes, Bâtiment 630, Unité
Mixte de Recherche-Centre National de la Recherche Scientifique 8618, Université Paris-Sud (XI), 91405 Orsay cedex, France
The recent publication of the complete sequence of the
Arabidopsis genome allowed us to identify and characterize the last two
members of the SHAGGY-like kinase (AtSK) gene family. As
a result, the study of the overall spatio-temporal organization of the
whole AtSK family in Arabidopsis has become an
achievable and necessary aim to understand the role of each SHAGGY-like
kinase during plant development. An analysis of the transcript level of
the 10 members of the family has been performed using the technique of
real-time quantitative reverse transcriptase-polymerase chain reaction.
Transcript levels in several organs, under different growth conditions,
were analyzed. To calibrate the results obtained, a number of other
genes, such as those coding for the two MAP3K s and the two
MAP4K s, as well as the stress response marker RD29A; the small
subunit of the Rubisco photosynthetic enzyme Ats1A; the MEDEA chromatin
remodeling factor; and the SCARECROW, ASYMMETRIC LEAVES 1, and SUPERMAN
transcription factors all involved in key steps of plant development
were used. The analysis of our data revealed that eight of the 10 genes
of the AtSK family displayed a pseudo-constitutive
expression pattern at the organ level. Conversely, AtSK13 responded to osmotic changes and saline
treatment, whereas AtSK31 was flower specific and
responded to osmotic changes and darkness.
1
This work was supported by the "Pluriformation
Genome" (contribution toward the purchasing of the real-time PCR apparatus).
*
Corresponding author; e-mail charrier{at}ibp.u-psud.fr; fax
33-1-69-15-34-25.
© 2002 American Society of Plant Physiologists
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