First published online October 3, 2002; 10.1104/pp.006783
Plant Physiol, October 2002, Vol. 130, pp. 618-626
Tomato Plants Ectopically Expressing Arabidopsis CBF1 Show
Enhanced Resistance to Water Deficit Stress1
Tsai-Hung
Hsieh,
Jent-turn
Lee,
Yee-yung
Charng, and
Ming-Tsair
Chan*
Institute of BioAgricultural Sciences, Academia Sinica,
Nankang, Taipei, 115, Taiwan, Republic of China (T.-H.H., J.-t.L.,
Y.-y.C., M.-T.C.); and National Graduate Institute of Life Sciences,
National Defense Medical Center, 114, Taiwan, Republic of China
(T.-H.H.)
A DNA cassette containing an Arabidopsis C
repeat/dehydration-responsive element binding factor 1 (CBF1) cDNA and a nos terminator, driven
by a cauliflower mosaic virus 35S promoter, was transformed into
the tomato (Lycopersicon esculentum) genome. These
transgenic tomato plants were more resistant to water deficit stress
than the wild-type plants. The transgenic plants exhibited growth
retardation by showing dwarf phenotype, and the fruit and seed numbers
and fresh weight of the transgenic tomato plants were apparently less than those of the wild-type plants. Exogenous gibberellic acid treatment reversed the growth retardation and enhanced growth of
transgenic tomato plants, but did not affect the level of water deficit
resistance. The stomata of the transgenic CBF1 tomato plants closed more rapidly than the wild type after water deficit treatment with or without gibberellic acid pretreatment. The transgenic tomato plants contained higher levels of Pro than those of the wild-type plants under normal or water deficit conditions. Subtractive hybridization was used to isolate the responsive genes to heterologous CBF1 in transgenic tomato plants and the
CAT1 (CATALASE1) was characterized.
Catalase activity increased, and hydrogen peroxide concentration
decreased in transgenic tomato plants compared with the wild-type
plants with or without water deficit stress. These results indicated
that the heterologous Arabidopsis CBF1 can confer water
deficit resistance in transgenic tomato plants.
1
This work was supported by Academia Sinica
(grant) and by the National Science Council of the Republic of China
(grant no. NSC-90-2311-B-001-071).
*
Corresponding author; e-mail mbmtchan{at}ccvax.sinica.edu.tw;
fax 886-2-26511164.
© 2002 American Society of Plant Physiologists
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