First published online October 3, 2002; 10.1104/pp.005538
Plant Physiol, October 2002, Vol. 130, pp. 666-674
Molecular Characterization of the Cotton GhTUB1 Gene
That Is Preferentially Expressed in Fiber1
Xue-Bao
Li,*
Lin
Cai,
Ning-Hui
Cheng,2 and
Jian-Wei
Liu3
Temasek Life Sciences Laboratory, 1 Research Link, National
University of Singapore, Singapore 117604
Each fiber of cotton (Gossypium hirsutum) is
a single epidermal cell that rapidly elongates to 2.5 to 3.0 cm from
the ovule surface within about 16 d after anthesis. A large number
of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the
process of fiber development. To investigate gene expression patterns
in fiber, a cDNA, GhTUB1, encoding  -tubulin was
isolated from a cotton fiber cDNA library. The analyses of RNA
northern-blot hybridization and reverse transcriptase-polymerase chain
reaction demonstrated that GhTUB1 transcripts
preferentially accumulated at high levels in fiber, at low levels in
ovules at the early stage of cotton boll development, and at very low
levels in other tissues of cotton. The corresponding
GhTUB1 gene including the promoter region was isolated
by screening a cotton genomic DNA library. To demonstrate the
specificity of the GhTUB1 promoter, the 5'-flanking
region including the promoter and 5'-untranslated region was fused with
the -glucuronidase reporter gene. The expression of
the reporter chimera was examined in a large number of transgenic cotton plants. Histochemical assays demonstrated that
GhTUB1::-glucuronidase fusion genes were expressed preferentially at high levels in fiber and
primary root tip of 1- to 3-d-old seedlings and at low levels in other
tissues such as ovule, pollen, seedling cotyledon, and root basal
portion. The results suggested that the GhTUB1 gene may
play a distinct and required role in fiber development. In addition,
the GhTUB1 promoter may have great potential for cotton improvement by genetic engineering.
1
This work was supported by Delta and Pine Land
Co. and by the National Science and Technology Board (Republic of Singapore).
2
Present address: Baylor College of Medicine, Children's
Nutrition Research Center, Room 11004, 1100 Bates Street, Houston, TX 77030.
3
Present address: Syngenta Singapore Pte Ltd., 6 Temasek
Boulevard, 09-02 Suntec Tower 4, Singapore 038986.
*
Corresponding author; e-mail xbli{at}tll.org.sg; fax
65-6872-7007.
© 2002 American Society of Plant Physiologists
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