First published online October 3, 2002; 10.1104/pp.009993
Plant Physiol, October 2002, Vol. 130, pp. 709-719
ACTCAT, a Novel cis-Acting Element for Proline- and
Hypoosmolarity-Responsive Expression of the ProDH Gene
Encoding Proline Dehydrogenase in Arabidopsis1
Rie
Satoh,
Kazuo
Nakashima,
Motoaki
Seki,
Kazuo
Shinozaki, and
Kazuko
Yamaguchi-Shinozaki*
Biological Resources Division, Japan International Research Center
for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686,
Japan (R.S., K.N., K.Y.-S.); Institute of Biological Sciences,
University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572,
Japan (R.S., K.S.); Laboratory of Plant Molecular Biology, RIKEN
Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
(R.S., M.S., K.S.); and Plant Functional Genomics Group, RIKEN Genomic
Sciences Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
(M.S., K.S.)
Proline (Pro) is one of the most widely distributed
osmolytes in water-stressed plants. We previously isolated from
Arabidopsis a gene encoding Pro dehydrogenase (ProDH), a mitochondrial
enzyme involved in the first step of the conversion of Pro to glutamic acid. The ProDH gene in Arabidopsis is up-regulated by
rehydration after dehydration but is down-regulated by dehydration.
ProDH is also induced by L-Pro and
hypoosmolarity. The induction of ProDH expression under
rehydration seems to be caused by both accumulated Pro and
hypoosmolarity. We analyzed a DNA region that is located 5' to the
transcription start site (a promoter region) of ProDH to
identify cis-acting elements involved in L-Pro-induced and
hypoosmolarity-induced expression in transgenic tobacco
(Nicotiana tabacum) and Arabidopsis plants. We found
that a 9-bp sequence, ACTCATCCT, in the ProDH promoter
is necessary for the efficient expression of ProDH in
response to L-Pro and hypoosmolarity. Moreover, ACTCAT is a
core cis-acting element, which we have called Pro- or
hypoosmolarity-responsive element (PRE), that is necessary for
L-Pro-responsive and hypoosmolarity-responsive expression of ProDH. Microarray and RNA gel-blot analyses showed
that 21 L-Pro-inducible genes have the PRE sequences in
their promoter regions. These results indicate that the PRE sequence
play an important role in the L-Pro-responsive gene expression.
1
This work was supported in part by the Program
for the Promotion of Basic Research Activities for Innovative
Biosciences. R.S. was supported by the Cooperative System for
Supporting Priority Research of the Japan Science and Technology Corporation.
*
Corresponding author; e-mail kazukoys{at}jircas.affrc.go.jp; fax
81-298-38-6643.
© 2002 American Society of Plant Physiologists
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