First published online September 6, 2002; 10.1104/pp.009688
Plant Physiol, October 2002, Vol. 130, pp. 720-728
Transcription Profiling of the Early Gravitropic
Response in Arabidopsis Using High-Density Oligonucleotide
Probe Microarrays1,[w]
Nick
Moseyko,
Tong
Zhu,
Hur-Song
Chang,
Xun
Wang, and
Lewis J.
Feldman*
Department of Plant and Microbial Biology, University of
California, 111 Koshland Hall, Berkeley, California 94720-3102 (N.M.,
L.J.F.); and Torrey Mesa Research Institute, 3115 Merryfield Row, San
Diego, California 92121 (T.Z., H.-S.C., X.W.)
Studies of plant tropisms, the directed growth toward or
away from external stimuli such as light and gravity, began more than a
century ago. Yet biochemical, physiological, and especially molecular
mechanisms of plant tropic responses remain for the most part unclear.
We examined expression of 8,300 genes during early stages of the
gravitropic response using high-density oligonucleotide probe
microarrays. Approximately 1.7% of the genes represented on the array
exhibited significant expression changes within the first 30 min of
gravity stimulation. Among gravity-induced genes were a number of genes
previously implicated to be involved in gravitropism. However, a much
larger number of the identified genes have not been previously
associated with gravitropism. Because reorientation of plants may also
expose plants to mechanical perturbations, we also compared the effects
of a gentle mechanical perturbation on mRNA levels during the gravity
response. It was found that approximately 39% of apparently
gravity-regulated genes were also regulated by the mechanical
perturbation caused by plant reorientation. Our study revealed the
induction of complex gene expression patterns as a consequence of
gravitropic reorientation and points to an interplay between the
gravitropic and mechanical responses and to the extreme sensitivity of
plants to even very gentle mechanical perturbations.
1
This work was supported by the National
Aeronautics and Space Administration (grant no. 98-HEDS-02) and by
the Torrey Mesa Research Institute.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail feldman{at}nature.berkeley.edu; fax
510-642-4995.
© 2002 American Society of Plant Physiologists
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