First published online September 20, 2002; 10.1104/pp.008680
Plant Physiol, October 2002, Vol. 130, pp. 770-783
Transcriptional and Posttranscriptional Regulation of Arabidopsis
TCH4 Expression by Diverse Stimuli. Roles of cis
Regions and Brassinosteroids1
Emanuil A.
Iliev,
Wei
Xu,2
Diana H.
Polisensky,
Man-Ho
Oh,3
Rebecca S.
Torisky,4
Steven D.
Clouse, and
Janet
Braam*
Biochemistry and Cell Biology, Rice University, Houston, Texas
77251-1892 (E.A.I., W.X., D.H.P., J.B.); and Department of
Horticultural Science, North Carolina State University, Raleigh, North
Carolina 27695 (M.-H.O., R.S.T., S.D.C.)
The Arabidopsis TCH4 gene is up-regulated in
expression by diverse environmental and hormonal stimuli. Because
TCH4 encodes a xyloglucan
endotransglucosylase/hydrolase, this change in expression may reflect a
recruitment of cell wall-modifying activity in response to
environmental stress and growth. How diverse stimuli lead to the common
response of TCH4 expression regulation is not known. Here, we show that induction of expression by the diverse stimuli of
touch, darkness, cold, heat, and brassinosteroids (BRs) is conferred to
reporter genes by the same 102-bp 5'-untranscribed TCH4
region; this result is consistent with the idea that shared regulatory
elements are employed by diverse stimuli. Distal regions influence
magnitude and kinetics of expression and likely harbor regulatory
elements that are redundant with those located more proximal to the
transcriptional start site. Substitution of the proximal regulatory
region sequences in the context of distal elements does not disrupt
inducible expression. TCH4 expression induction is
transcriptional, at least in part because 5'-untranscribed sequences
are sufficient to confer this regulation. However, 5'-untranslated sequences are necessary and sufficient to confer the marked transience of TCH4 expression, most likely through an effect on
mRNA stability. Perception of BR is not necessary for
TCH4::GUS induction by environmental stimuli
because regulation is intact in the BR-insensitive mutant, bri1-2. The full response to auxin, however, requires
the functioning of BRI1. Developmental expression of
TCH4 is unlikely to be meditated by BR because
TCH4::GUS is expressed in BR perception and
biosynthetic mutants bri1-2 and det2-1, respectively.
1
This work was supported by the National Science
Foundation (grant no. IBN9982654 to J.B.), by the Department of Energy
(grant no. DE-FG03-99ER20331 to J.B.), in part by the National
Institutes of Health (Biotechnology Training Grant no. T32-Gm08362 to
E.A.I.), by the National Science Foundation (Integrative Biology and
Neuroscience; Integrative Plant Biology, to S.D.C.'s
laboratory), by the U.S. Department of Agriculture/National Research
Initiative (Plant Growth and Development, to S.D.C.'s laboratory), and
by the North Carolina Agricultural Research Service (to S.D.C.'s laboratory).
2
Present address: Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, CA 94588.
3
Present address: TS Corporation R&D Center, 6-14, 1ka,
Buksung-Dong, Jung-Gu, Incheon 400-201, Korea.
4
Present address: Sanford Scientific, Inc., 877 Marshall Road, Waterloo, NY 13165.
*
Corresponding author; e-mail braam{at}bioc.rice.edu; fax
713-348-5154.
© 2002 American Society of Plant Physiologists
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