First published online September 6, 2002; 10.1104/pp.005272
Plant Physiol, October 2002, Vol. 130, pp. 887-894
An Arabidopsis Mutant Defective in Jasmonate Response Is Allelic
to the Auxin-Signaling Mutant axr11
Iskender
Tiryaki2 and
Paul E.
Staswick*
Department of Agronomy and Horticulture, University of Nebraska,
Lincoln, Nebraska 68583-0915
A screen for Arabidopsis mutants that were insensitive to methyl
jasmonate (MeJA) in an assay for seedling root growth yielded only
alleles of previously isolated mutants jar1 and
coi1, with one exception. Mapping of the locus and
morphological characterization of the new mutant suggested it might be
allelic to axr1, which had not previously been reported
to show resistance to MeJA. The F1 from a cross of the new
mutant with axr1-3 did not show complementation, confirming that these are the same genes. The new allele is called axr1-24. In addition to MeJA and indole-3-acetic acid
(IAA), axr1-24 had decreased sensitivity to
1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine,
epi-brassinolide, and abscisic acid. Both axr1-24 and
the previously characterized axr1-3 allele were shown to
be susceptible to the opportunistic pathogen Pythium irregulare, a trait found in other jasmonate response mutants, including jar1-1. The double mutant
jar1-1/axr1-3 was more resistant to
inhibition of root growth by MeJA and was more susceptible to P.
irregulare infection than either single mutant, suggesting these genes might act in independent response pathways. In contrast, resistance to IAA in the double mutant was not different from axr1-3. Northern-blot analysis showed that IAA induced
the jasmonate-responsive lipoxygenase 2,
AOS, and AtVSP gene transcripts and
induction was strongly impaired in axr1-3. However,
transcript induction by MeJA was only minimally affected in
axr1-3. This study demonstrates that in addition to
auxin signaling, the AXR1 locus is involved in MeJA
response, providing a mechanistic link between jasmonate and
auxin-signaling pathways.
1
This work was supported by the Nebraska Research
Initiative and by the University of Nebraska Center for Biotechnology.
This paper is a contribution of the University of Nebraska Agricultural Research Division (Lincoln). This is journal series no. 13,647.
2
Present address: Department of Agronomy, Kahramanmaras
Sutcu Imam University, Kahramanmaras, 46060 Turkey.
*
Corresponding author; e-mail pstaswick1{at}unl.edu; fax
402-472-7904.
© 2002 American Society of Plant Physiologists
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