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Plant Physiol, November 2002, Vol. 130, pp. 1132-1142
LeCTR1, a Tomato CTR1-Like Gene,
Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel
Expression Patterns in Tomato1
Julie
Leclercq,
Lori C.
Adams-Phillips,
Hicham
Zegzouti,
Brian
Jones,
Alain
Latché,
James J.
Giovannoni,
Jean-Claude
Pech, and
Mondher
Bouzayen*
Unité Mixte de Recherche 990, Institut
National de la Recherche Agronomique/Institut National
Polytechnique-Ecole Nationale Supérieure Agronomique, Boite
Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L.,
H.Z., B.J., A.L., J.-C.P., M.B.); and United States Department of
Agriculture-Agricultural Research Service and Boyce Thompson Institute
for Plant Research, Tower Road, Ithaca, New York 14853 (L.C.A.-P.,
J.J.G.)
LeCTR1 was initially isolated by both
differential display reverse transcriptase-polymerase chain reaction
screening for tomato (Lycopersicon esculentum)
fruit ethylene-inducible genes and through homology with the
Arabidopsis CTR1 cDNA. LeCTR1 shares strong nucleotide sequence homology with Arabidopsis CTR1, a
gene acting downstream of the ethylene receptor and showing similarity
to the Raf family of serine/threonine protein kinases. The length of
the LeCTR1 transcribed region from ATG to stop codon
(12,000 bp) is more than twice that of Arabidopsis CTR1
(4,700 bp). Structural analysis reveals perfect conservation of both
the number and position of introns and exons in LeCTR1
and Arabidopsis CTR1. The introns in
LeCTR1 are much longer, however. To address whether this
structural conservation is indicative of functional conservation of the
corresponding proteins, we expressed LeCTR1 in the
Arabidopsis ctr1-1 (constitutive triple response
1) mutant under the direction of the 35S promoter. Our data
clearly show that ectopic expression of LeCTR1 in the Arabidopsis ctr1-1 mutant can restore normal ethylene
signaling. The recovery of normal ethylene sensitivity upon
heterologous expression of LeCTR1 was also confirmed by
restored glucose sensitivity absent in the Arabidopsis
ctr1-1 mutant. Expression studies confirm ethylene
responsiveness of LeCTR1 in various tissues, including ripening fruit, and may suggest the evolution of alternate regulatory mechanisms in tomato versus Arabidopsis.
1
This work was supported in part by the European
Union (grant no. FAIR CT-95 0225), by the Institut National de
la Recherche Agronomique (Action Transversale Structurante
Tomate, grant no. 2001-2003), by the "Midi-Pyrénées"
Regional Council (grant nos. 99009080 and 01002710 to M.B.), and by the
National Science Foundation (grant nos. IBN-9604115 and DBI-9872617
to J.J.G.). This research forms part of the requirement for the degree
of PhD for J.L.
*
Corresponding author; e-mail bouzayen{at}ensat.fr; fax
33-5-62-19-35-73.
© 2002 American Society of Plant Biologists
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