First published online October 17, 2002; 10.1104/pp.007179
Plant Physiol, November 2002, Vol. 130, pp. 1152-1161
Isolation and Characterization of a Rice Dwarf Mutant with a
Defect in Brassinosteroid Biosynthesis1
Masaki
Mori,*
Takahito
Nomura,
Hisako
Ooka,
Masumi
Ishizaka,
Takao
Yokota,
Kazuhiko
Sugimoto,
Ken
Okabe,2
Hideyuki
Kajiwara,
Kouji
Satoh,
Koji
Yamamoto,
Hirohiko
Hirochika, and
Shoshi
Kikuchi
Departments of Molecular Genetics (M.M., H.O., Ka.S., K.O., Ko.S.,
H.H., S.K.) and Biochemistry (H.K.), National Institute of
Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan; Department
of Biosciences, Teikyo University, Utsunomiya, Tochigi 320-8551, Japan
(T.N., T.Y.); Nagaoka University of Technology, Nagaoka, Niigata
940-2137, Japan (H.O., K.Y.); and Chemical Analysis Research Center,
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki
305-8604, Japan (M.I.)
We have isolated a new recessive dwarf mutant of rice (Oryza
sativa L. cv Nipponbare). Under normal growth conditions, the mutant has very short leaf sheaths; has short, curled, and frizzled leaf blades; has few tillers; and is sterile. Longitudinal sections of
the leaf sheaths revealed that the cell length along the longitudinal axis is reduced, which explains the short leaf sheaths. Transverse sections of the leaf blades revealed enlargement of the motor cells
along the dorsal-ventral axis, which explains the curled and frizzled
leaf blades. In addition, the number of crown roots was smaller and the
growth of branch roots was weaker than those in the wild-type plant.
Because exogenously supplied brassinolide considerably restored the
normal phenotypes, we designated the mutant
brassinosteroid-dependent 1
(brd1). Further, under darkness, brd1
showed constitutive photomorphogenesis. Quantitative analyses of
endogenous sterols and brassinosteroids (BRs) indicated that BR-6-oxidase, a BR biosynthesis enzyme, would be defective. In fact, a
0.2-kb deletion was detected in the genomic region of OsBR6ox (a rice BR-6-oxidase gene) in the
brd1 mutant. These results indicate that BRs are
involved in many morphological and physiological processes in rice,
including the elongation and unrolling of leaves, development of
tillers, skotomorphogenesis, root differentiation, and reproductive
growth, and that the defect of BR-6-oxidase caused the
brd1 phenotype.
1
This work was supported by the Ministry of
Agriculture, Forestry, and Fisheries of Japan (rice genome project no.
MP-1202), by the Human Frontiers Science Program (grant no.
RG00162-2000 to T.Y.), and by the Japan Society for the Promotion of
Science (Grant-in-Aid for Scientific Research no. 11460057 to T.Y.).
T.N. is a research fellow of the Japan Society for the Promotion of Science since 1998.
2
Present address: Sakata Seed Co. Ltd., Quality Assurance
Division, 2-7-1, Nakamachidai, Tuduki, Yokohama 224-0041, Japan.
*
Corresponding author; e-mail morimasa{at}nias.affrc.go.jp; fax
81-298-38-7007.
© 2002 American Society of Plant Biologists
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