First published online October 15, 2002; 10.1104/pp.006007
Plant Physiol, November 2002, Vol. 130, pp. 1213-1220
Induction of Jasmonate Biosynthesis in Arbuscular Mycorrhizal
Barley Roots1,2
Bettina
Hause,*
Walter
Maier,
Otto
Miersch,
Robert
Kramell, and
Dieter
Strack
Abteilung Sekundärstoffwechsel (B.H., W.M., D.S.) and
Abteilung Naturstoff-Biotechnologie (O.M., R.K.), Leibniz-Institut
für Pflanzenbiochemie, D-06120 Halle (Saale), Germany
Colonization of barley (Hordeum vulgare cv
Salome) roots by an arbuscular mycorrhizal fungus, Glomus
intraradices Schenck & Smith, leads to elevated levels of
endogenous jasmonic acid (JA) and its amino acid conjugate
JA-isoleucine, whereas the level of the JA precursor, oxophytodienoic
acid, remains constant. The rise in jasmonates is accompanied by the
expression of genes coding for an enzyme of JA biosynthesis (allene
oxide synthase) and of a jasmonate-induced protein (JIP23). In situ
hybridization and immunocytochemical analysis revealed that expression
of these genes occurred cell specifically within arbuscule-containing
root cortex cells. The concomitant gene expression indicates that
jasmonates are generated and act within arbuscule-containing cells. By
use of a near-synchronous mycorrhization, analysis of temporal
expression patterns showed the occurrence of transcript accumulation 4 to 6 d after the appearance of the first arbuscules. This suggests that the endogenous rise in jasmonates might be related to the fully
established symbiosis rather than to the recognition of interacting
partners or to the onset of interaction. Because the plant supplies the
fungus with carbohydrates, a model is proposed in which the induction
of JA biosynthesis in colonized roots is linked to the stronger sink
function of mycorrhizal roots compared with nonmycorrhizal roots.
1
This work was supported by the Deutsche
Forschungsgemeinschaft (grant no. HA2655/4-1 in SPP1084) and by Fonds
der Chemischen Industrie.
2
This paper is dedicated to Prof. Dr. Benno Parthier on
the occasion of his 70th birthday.
*
Corresponding author; e-mail bhause{at}ipb-halle.de; fax
49-345-55821219.
© 2002 American Society of Plant Biologists
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