|
Plant Physiol, November 2002, Vol. 130, pp. 1241-1253
Characterization of AtCDC48. Evidence for Multiple Membrane
Fusion Mechanisms at the Plane of Cell Division in Plants1
David M.
Rancour,
Carrie E.
Dickey,2
Sookhee
Park, and
Sebastian Y.
Bednarek*
Department of Biochemistry, University of Wisconsin, 433 Babcock
Drive, Madison, Wisconsin 53706
The components of the cellular machinery that accomplish the
various complex and dynamic membrane fusion events that occur at the
division plane during plant cytokinesis, including assembly of
the cell plate, are not fully understood. The most well-characterized component, KNOLLE, a cell plate-specific soluble
N-ethylmaleimide-sensitive fusion protein (NSF)-attachment
protein receptor (SNARE), is a membrane fusion machine component
required for plant cytokinesis. Here, we show the plant ortholog
of Cdc48p/p97, AtCDC48, colocalizes at the division plane in dividing
Arabidopsis cells with KNOLLE and another SNARE, the plant ortholog of
syntaxin 5, SYP31. In contrast to KNOLLE, SYP31 resides in defined
punctate membrane structures during interphase and is targeted during
cytokinesis to the division plane. In vitro-binding studies demonstrate
that AtCDC48 specifically interacts in an ATP-dependent manner with SYP31 but not with KNOLLE. In contrast, we show that KNOLLE assembles in vitro into a large approximately 20S complex in an
Sec18p/NSF-dependent manner. These results suggest that there are at
least two distinct membrane fusion pathways involving Cdc48p/p97 and
Sec18p/NSF that operate at the division plane to mediate plant
cytokinesis. Models for the role of AtCDC48 and SYP31 at the division
plane will be discussed.
1
This work was supported by the Department of
Energy, Division of Energy Biosciences (project no.
DE-FG02-99ER20332), by the U.S. Department of Agriculture-Plant
Growth and Development (project no. 98-35304-6671), by the Milwaukee
Foundation (award to S.Y.B.), and by the National Science
Foundation/Department of Energy/U.S. Department of Agriculture
Collaborative Research in Plant Biology Program (grant no. 9602222 to
C.E.D. and S.P.).
2
Present address: Stower's Institute for Medical
Research, 1000 E. 50th Street, Kansas City, MO 64110.
*
Corresponding author; e-mail bednarek{at}biochem.wisc.edu; fax
608-262-3453.
© 2002 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
J. Aker, R. Hesselink, R. Engel, R. Karlova, J. W. Borst, A. J.W.G. Visser, and S. C. de Vries
In Vivo Hexamerization and Characterization of the Arabidopsis AAA ATPase CDC48A Complex Using Forster Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy
Plant Physiology,
October 1, 2007;
145(2):
339 - 350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sanderfoot
Increases in the Number of SNARE Genes Parallels the Rise of Multicellularity among the Green Plants
Plant Physiology,
May 1, 2007;
144(1):
6 - 17.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Park, D. M. Rancour, and S. Y. Bednarek
Protein Domain-Domain Interactions and Requirements for the Negative Regulation of Arabidopsis CDC48/p97 by the Plant Ubiquitin Regulatory X (UBX) Domain-containing Protein, PUX1
J. Biol. Chem.,
February 23, 2007;
282(8):
5217 - 5224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. W. Han, C. A. Bingman, D. K. Mahnke, R. M. Bannen, S. Y. Bednarek, R. L. Sabina, and G. N. Phillips Jr.
Membrane Association, Mechanism of Action, and Structure of Arabidopsis Embryonic Factor 1 (FAC1)
J. Biol. Chem.,
May 26, 2006;
281(21):
14939 - 14947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Karlova, S. Boeren, E. Russinova, J. Aker, J. Vervoort, and S. de Vries
The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 Protein Complex Includes BRASSINOSTEROID-INSENSITIVE1
PLANT CELL,
March 1, 2006;
18(3):
626 - 638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Muller, P. Piffanelli, A. Devoto, M. Miklis, C. Elliott, B. Ortmann, P. Schulze-Lefert, and R. Panstruga
Conserved ERAD-Like Quality Control of a Plant Polytopic Membrane Protein
PLANT CELL,
January 1, 2005;
17(1):
149 - 163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Rancour, S. Park, S. D. Knight, and S. Y. Bednarek
Plant UBX Domain-containing Protein 1, PUX1, Regulates the Oligomeric Structure and Activity of Arabidopsis CDC48
J. Biol. Chem.,
December 24, 2004;
279(52):
54264 - 54274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. K. Peterman, Y. M. Ohol, L. J. McReynolds, and E. J. Luna
Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides
Plant Physiology,
October 1, 2004;
136(2):
3080 - 3094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Boonsirichai, J. C. Sedbrook, R. Chen, S. Gilroy, and P. H. Masson
ALTERED RESPONSE TO GRAVITY Is a Peripheral Membrane Protein That Modulates Gravity-Induced Cytoplasmic Alkalinization and Lateral Auxin Transport in Plant Statocytes
PLANT CELL,
November 1, 2003;
15(11):
2612 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-H. Kang, J. S. Busse, and S. Y. Bednarek
Members of the Arabidopsis Dynamin-Like Gene Family, ADL1, Are Essential for Plant Cytokinesis and Polarized Cell Growth
PLANT CELL,
April 1, 2003;
15(4):
899 - 913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
