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Plant Physiol, November 2002, Vol. 130, pp. 1406-1413
Interaction of Sulfate Assimilation with Carbon and Nitrogen
Metabolism in Lemna minor1
Stanislav
Kopriva,*
Marianne
Suter,
Peter
von Ballmoos,
Holger
Hesse,
Urs
Krähenbühl,
Heinz
Rennenberg, and
Christian
Brunold
Department of Tree Physiology, University of Freiburg, D-79085
Freiburg, Germany (S.K., H.R.); Institute of Plant Sciences, CH-3013
Berne, Switzerland (M.S., P.v.B., C.B.); Institute of Biology, Free
University of Berlin, Applied Genetics, D-14195 Berlin, Germany
(H.H.); and Department of Chemistry and Biochemistry, University of
Berne, CH-3012 Berne, Switzerland (U.K.)
Cysteine synthesis from sulfide and
O-acetyl-L-serine (OAS) is a reaction
interconnecting sulfate, nitrogen, and carbon assimilation. Using
Lemna minor, we analyzed the effects of omission of
CO2 from the atmosphere and simultaneous application of
alternative carbon sources on adenosine 5'-phosphosulfate reductase
(APR) and nitrate reductase (NR), the key enzymes of sulfate and
nitrate assimilation, respectively. Incubation in air without
CO2 led to severe decrease in APR and NR activities and
mRNA levels, but ribulose-1,5-bisphosphate carboxylase/oxygenase
was not considerably affected. Simultaneous addition of sucrose (Suc)
prevented the reduction in enzyme activities, but not in mRNA levels.
OAS, a known regulator of sulfate assimilation, could also attenuate the effect of missing CO2 on APR, but did not affect NR.
When the plants were subjected to normal air after a 24-h pretreatment in air without CO2, APR and NR activities and mRNA levels
recovered within the next 24 h. The addition of Suc and glucose in
air without CO2 also recovered both enzyme activities, with
OAS again influenced only APR.
35SO42 feeding showed that
treatment in air without CO2 severely inhibited sulfate
uptake and the flux through sulfate assimilation. After a resupply of
normal air or the addition of Suc, incorporation of 35S
into proteins and glutathione greatly increased. OAS treatment resulted
in high labeling of cysteine; the incorporation of 35S in
proteins and glutathione was much less increased compared with
treatment with normal air or Suc. These results corroborate the tight
interconnection of sulfate, nitrate, and carbon assimilation.
1
This work was supported by the Swiss National
Foundation (grant no. 3149246-96 to C.B.).
*
Corresponding author; e-mail Stanislav.Kopriva{at}ctp.uni-freiburg.de;
fax 49-761-2038302.
© 2002 American Society of Plant Biologists
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