First published online October 15, 2002; 10.1104/pp.009951
Plant Physiol, November 2002, Vol. 130, pp. 1454-1463
Induction of the Arginine Decarboxylase ADC2 Gene
Provides Evidence for the Involvement of Polyamines in the Wound
Response in Arabidopsis1
Miguel A.
Perez-Amador,*
Jose
Leon,
Pamela J.
Green,2 and
Juan
Carbonell
Instituto de Biología Molecular y Celular de Plantas,
Universidad Politécnica de Valencia, Consejo Superior
de Investigaciones Científicas, 46022 Valencia, Spain
(M.A.P.-A., J.L., J.C.); and Department of Energy, Plant Research
Laboratory, and Department of Biochemistry, Michigan State University,
East Lansing, Michigan 48824 (P.J.G.)
Polyamines are small ubiquitous molecules that have been
involved in nearly all developmental processes, including the
stress response. Nevertheless, no direct evidence of a role of
polyamines in the wound response has been described. We have studied
the expression of genes involved in polyamine biosynthesis in response to mechanical injury. An increase in the expression of the arginine decarboxylase 2 (ADC2) gene in response to mechanical
wounding and methyl jasmonate (JA) treatment in Arabidopsis was
detected by using DNA microarray and RNA gel-blot analysis. No
induction was observed for the ADC1 gene or other genes
coding for spermidine and spermine synthases, suggesting that
ADC2 is the only gene of polyamine biosynthesis involved
in the wounding response mediated by JA. A transient increase in the
level of free putrescine followed the increase in the mRNA level for
ADC2. A decrease in the level of free spermine,
coincident with the increase in putrescine after wounding, was also
observed. Abscisic acid effected a strong induction on
ADC2 expression and had no effect on ADC1
expression. Wound-induction of ADC2 mRNA was not
prevented in the JA-insensitive coi1 mutant. The
different pattern of expression of ADC2 gene in wild-type and coi1 mutant might be due to the dual regulation of
ADC2 by abscisic acid and JA signaling pathways.
This is the first direct evidence of a function of polyamines in the
wound-response, and it opens a new aspect of polyamines in plant
biology.
1
This work was supported by the Plan Nacional de
I+D (Spain; grant nos. BIO99-1129 to J.L. and BIO99-1201-C02-01 to
J.C.).
2
Present address: Department of Plant and Soil Sciences,
Delaware Biotechnology Institute, College of Marine Studies, University of Delaware, 15 Innovation Way, Newark, DE 19711.
*
Corresponding author; e-mail mpereza{at}ibmcp.upv.es; fax
34-96-3877859.
© 2002 American Society of Plant Biologists
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