First published online November 7, 2002; 10.1104/pp.012179
Plant Physiol, December 2002, Vol. 130, pp. 1626-1635
Contiguous Genomic DNA Sequence Comprising the 19-kD Zein
Gene Family from Maize1
Rentao
Song and
Joachim
Messing*
Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854-8020
A new approach has been undertaken to analyze the sequences
and linear organization of the 19-kD zein genes in maize (Zea mays). A high-coverage, large-insert genomic library of the
inbred line B73 based on bacterial artificial chromosomes was used to isolate a redundant set of clones containing members of the 19-kD zein
gene family, which previously had been estimated to consist of 50 members. The redundant set of clones was used to create bins of
overlapping clones that represented five distinct genomic regions.
Representative clones containing the entire set of 19-kD zein genes
were chosen from each region and sequenced. Seven bacterial artificial
chromosome clones yielded 1,160 kb of genomic DNA. Three of them formed
a contiguous sequence of 478 kb, the longest contiguous sequenced
region of the maize genome. Altogether, these DNA sequences provide the
linear organization of 25 19-kD zein genes, one-half the number
previously estimated. It is suggested that the difference is because of
haplotypes exhibiting different degrees of gene amplification in the
zein multigene family. About one-half the genes present in B73 appear
to be expressed. Because some active genes have only been duplicated
recently, they are so conserved in their sequence that previous cDNA
sequence analysis resulted in "unigenes" that were actually derived
from different gene copies. This analysis also shows that the 22- and
19-kD zein gene families shared a common ancestor. Although both
ancestral genes had the same incremental gene amplification, the 19-kD
zein branch exhibited a greater degree of far-distance gene
translocations than the 22-kD zein gene family.
1
This work was supported by the Department of
Energy (grant no. DE-FG05-95ER20194 to J.M.).
*
Corresponding author; e-mail messing{at}mbcl.rutgers.edu; fax
732-445-0072.
© 2002 American Society of Plant Biologists
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