First published online November 21, 2002; 10.1104/pp.014951
Plant Physiol, December 2002, Vol. 130, pp. 1728-1738
Comparative Sequence Analysis of the Sorghum Rph
Region and the Maize Rp1 Resistance Gene Complex
Wusirika
Ramakrishna,
John
Emberton,
Phillip
SanMiguel,
Matthew
Ogden,
Victor
Llaca,
Joachim
Messing, and
Jeffrey L.
Bennetzen*
Department of Biological Sciences, Purdue University, West
Lafayette, Indiana 47907 (W.R., J.E., M.O., J.L.B.); Purdue University
Genomics Core, Purdue University, West Lafayette, Indiana 47907 (P.S.M.); and Waksman Institute, Rutgers University, Piscataway, New
Jersey 08854 (V.L., J.M.)
A 268-kb chromosomal segment containing sorghum
(Sorghum bicolor) genes that are orthologous to the
maize (Zea mays) Rp1 disease resistance (R) gene complex was sequenced. A region of approximately 27 kb in sorghum was found to contain five Rp1 homologs,
but most have structures indicating that they are not functional. In
contrast, maize inbred B73 has 15 Rp1 homologs in two
nearby clusters of 250 and 300 kb. As at maize Rp1, the
cluster of R gene homologs is interrupted by the presence of several
genes that appear to have no resistance role, but these genes were
different from the ones found within the maize Rp1
complex. More than 200 kb of DNA downstream from the sorghum
Rp1-orthologous R gene cluster was sequenced and found
to contain many duplicated and/or truncated genes. None of the
duplications currently exist as simple tandem events, suggesting that
numerous rearrangements were required to generate the current genomic
structure. Four truncated genes were observed, including one gene that
appears to have both 5' and 3' deletions. The maize Rp1
region is also unusually enriched in truncated genes. Hence, the
orthologous maize and sorghum regions share numerous structural
features, but all involve events that occurred independently in each
species. The data suggest that complex R gene clusters are unusually
prone to frequent internal and adjacent chromosomal rearrangements of
several types.
*
Corresponding author; e-mail maize{at}bilbo.bio.purdue.edu;
fax 765-496-1496.
© 2002 American Society of Plant Biologists
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