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Plant Physiol, December 2002, Vol. 130, pp. 1827-1836
Ectopic Expression of Carpel-Specific MADS Box Genes from Lily
and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in
Arabidopsis1
Tsai-Yu
Tzeng,2
Hsing-Yu
Chen,2 and
Chang-Hsien
Yang*
Graduate Institute of Biotechnology, National Chung Hsing
University, Taichung, Taiwan 40227, Republic of China
Two MADS box genes, Lily MADS Box Gene 2
(LMADS2) and Eustoma grandiflorum MADS Box Gene
1 (EgMADS1), with an extensive similarity to the
petunia (Petunia hybrida) FLORAL BINDING PROTEIN
7/11 and Arabidopsis AGL11, were
characterized from the lily (Lilium longiflorum) and
lisianthus (Eustoma grandiflorum). The expression of
LMADS2 and EgMADS1 mRNA was restricted to
the carpel and was absent in the other flower organs or vegetative
leaves. LMADS2 mRNA was detected mainly in ovules and
weakly in style tissues of the carpel, whereas EgMADS1
mRNA was only expressed in the ovules. Transgenic Arabidopsis plants
ectopically expressing LMADS2 or EgMADS1
showed similar novel phenotypes resembling
35S::AGAMOUS plants by
significantly reducing plant size, flowering early, and losing
inflorescence indeterminacy. Ectopic expression of these two genes also
generated similar ap2-like flowers by inducing homeotic
conversion of the sepals into carpel-like structures in which stigmatic
papillae and ovules were observed. In addition, the petals were
converted into stamen-like structures in the second whorl of
35S::LMADS2 and
35S::EgMADS1 transgenic
Arabidopsis. Our data indicated that LMADS2 and
EgMADS1 are putative D functional MADS box genes in lily
and lisianthus with a function similar to C functional genes once
ectopically expressed in Arabidopsis.
1
This work was supported by the Council of
Agriculture and National Science Council (Taiwan, Republic of China;
grant nos. 90AS-2.1.1-FD-Z1 and NSC89-2311-B-005-050 to
C.-H.Y.).
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail chyang{at}dragon.nchu.edu.tw; fax
886-4-2285-3126/3527.
© 2002 American Society of Plant Biologists
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