First published online November 7, 2002; 10.1104/pp.010587
Plant Physiol, December 2002, Vol. 130, pp. 1974-1982
Auxin Regulation of the Gibberellin Pathway in Pea1
Damian P.
O'Neill and
John
J.
Ross*
School of Plant Science, University of Tasmania, Hobart, Tasmania
7001, Australia
The auxin indole-3-acetic acid (IAA) has been shown to promote the
biosynthesis of the active gibberellin (GA1) in shoots of
pea (Pisum sativum). We used northern analysis to
investigate the timing of IAA-induced changes in transcript levels of
PsGA3ox1 (Mendel's LE),
PsGA2ox1, PsGA2ox2, and
PsGA20ox1, key genes for the later stages of
GA1 biosynthesis and metabolism in pea. Rapid (2-4 h)
changes occurred in the transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2 after
treatent with IAA. [14C]GA1 metabolism
studies in decapitated shoots indicated that IAA inhibits
GA1 deactivation, consistent with the suppression of
PsGA2ox1 (SLN) transcript levels by IAA.
Studies with the sln mutant also indicated that
PsGA2ox1 activity is involved in GA1 deactivation in decapitated shoots. Culture of excised internode tissue
in the presence of auxin clearly demonstrated that internode tissue is
a site of GA1 biosynthesis per se. Excised internode tissue
cultured in the presence/absence of cycloheximide showed that de novo
protein synthesis is required for IAA-induced increases in
PsGA3ox1 transcript levels. Auxin dose response studies
indicated that IAA concentration is a critical determinant of
GA1 biosynthesis over 1 to 2 orders of magnitude, and a
range of auxins was shown to affect the GA pathway.
1
This work was supported by the Australian
Research Council.
*
Corresponding author; e-mail John.Ross{at}utas.edu.au; fax
61-3-62-262698.
© 2002 American Society of Plant Biologists
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