|
Plant Physiol, December 2002, Vol. 130, pp. 2177-2187
The Elicitor Cryptogein Blocks Glucose Transport in Tobacco
Cells1
Stéphane
Bourque,
Rémi
Lemoine,
Anabelle
Sequeira-Legrand,
Léon
Fayolle,
Serge
Delrot, and
Alain
Pugin*
Unité Mixte de Recherche-Institut National de la Recherche
Agronomique/Université de Bourgogne, Biochimie, Biologie
Cellulaire, et Ecologie des Interactions Plantes/Micro-organismes, 17 Rue Sully, BV 86510-21065 Dijon cedex, France (S.B., L.F., A.P.);
Institut Universitaire de la Vigne et du Vin Jules Guyot,
Université de Bourgogne, 21004 Dijon cedex, France (S.B.);
Unité Mixte de Recherche-Centre National de la Recherche
Scientifique 6161, Laboratoire de Physiologie et Biochimie
Végétales, Université de Poitiers, Unité de
Formation et de Recherche Sciences, 40 Avenue du Recteur
Pineau, 86022 Poitiers cedex, France (R.L., S.D.); and Centre Commun de
Cytométrie en Flux et de Tri Cellulaire, Université de
Bourgogne, Boite Postale 138, 21004 Dijon cedex, France
(A.S.-L.)
Cryptogein is a 10-kD protein secreted by the oomycete
Phytophthora cryptogea that induces a hypersensitive
response on tobacco (Nicotiana tabacum var. Xanthi)
plants and a systemic acquired resistance against various pathogens.
The mode of action of this elicitor has been studied using tobacco cell
suspensions. Our previous data indicated that within minutes,
cryptogein signaling involves various events including changes in ion
fluxes, protein phosphorylation, sugar metabolism, and, eventually,
cell death. These results suggested that transport of sugars could be
affected and, thus, involved in the complex relationships between plant and microorganisms via elicitors. This led us to investigate the effects of cryptogein on glucose (Glc) uptake and mitochondrial activity in tobacco cells. Cryptogein induces an immediate inhibition of Glc uptake, which is not attributable to plasma membrane (PM) depolarization. Conversely, cryptogein-induced valine uptake is because
of PM depolarization. Inhibition of the PM Glc transporter(s) was shown
to be mediated by a calcium-dependent phosphorylation process, and is
independent of active oxygen species production. This inhibition was
associated with a strong decrease in O2 uptake rate by
cells and a large mitochondrial membrane depolarization. Thus,
inhibition of Glc uptake accompanied by inhibition of phosphorylative oxidation may participate in hypersensitive cell death. These results
are discussed in the context of competition between plants and
microorganisms for apoplastic sugars.
1
This work was supported by the Ministère
de l'Education Nationale, de la Recherche, et de la Technologie, by
the Institut National de la Recherche Agronomique, and by the Conseil
Régional de Bourgogne.
*
Corresponding author; e-mail pugin{at}dijon.inra.fr; fax
33-3-80-69-32-26.
© 2002 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
H. Azevedo, T. Lino-Neto, and R. M. Tavares
The Necrotroph Botrytis cinerea Induces a Non-Host Type II Resistance Mechanism in Pinus pinaster Suspension-Cultured Cells
Plant Cell Physiol.,
March 1, 2008;
49(3):
386 - 395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Horemans, T. Raeymaekers, K. Van Beek, A. Nowocin, R. Blust, K. Broos, A. Cuypers, J. Vangronsveld, and Y. Guisez
Dehydroascorbate uptake is impaired in the early response of Arabidopsis plant cell cultures to cadmium
J. Exp. Bot.,
December 1, 2007;
58(15-16):
4307 - 4317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology,
August 1, 2006;
141(4):
1563 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bae, M. S. Kim, R. C. Sicher, H.-J. Bae, and B. A. Bailey
Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis
Plant Physiology,
July 1, 2006;
141(3):
1056 - 1067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Azevedo, C. Conde, H. Geros, and R. M. Tavares
The Non-host Pathogen Botrytis cinerea Enhances Glucose Transport in Pinus pinaster Suspension-cultured Cells
Plant Cell Physiol.,
February 1, 2006;
47(2):
290 - 298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot.,
May 1, 2005;
56(415):
1409 - 1418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Lamotte, K. Gould, D. Lecourieux, A. Sequeira-Legrand, A. Lebrun-Garcia, J. Durner, A. Pugin, and D. Wendehenne
Analysis of Nitric Oxide Signaling Functions in Tobacco Cells Challenged by the Elicitor Cryptogein
Plant Physiology,
May 1, 2004;
135(1):
516 - 529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Vacca, M. C. de Pinto, D. Valenti, S. Passarella, E. Marra, and L. De Gara
Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells
Plant Physiology,
March 1, 2004;
134(3):
1100 - 1112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Peiter and S. Schubert
Sugar uptake and proton release by protoplasts from the infected zone of Vicia faba L. nodules: evidence against apoplastic sugar supply of infected cells
J. Exp. Bot.,
July 1, 2003;
54(388):
1691 - 1700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
