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Plant Physiol, January 2003, Vol. 131, pp. 102-113

The Sucrose Transporter StSUT1 Localizes to Sieve Elements in Potato Tuber Phloem and Influences Tuber Physiology and Development1,[w]

Christina Kühn,* Mohammad-Reza Hajirezaei, Alisdair R. Fernie, Ute Roessner-Tunali, Tomasz Czechowski, Brigitte Hirner,2 and Wolf B. Frommer

Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany (C.K., B.H., W.B.F.); Institut für Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, D-06466 Gatersleben, Germany (M.-R.H.); and Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Golm, Germany (A.R.F., U.R.-T., T.C.)

The sucrose (Suc) H+-cotransporter StSUT1 from potato (Solanum tuberosum), which is essential for long-distance transport of Suc and assumed to play a role in phloem loading in mature leaves, was found to be expressed in sink tubers. To answer the question of whether SUT1 serves a function in phloem unloading in tubers, the promoter was fused to gusA and expression was analyzed in transgenic potato. SUT1 expression was unexpectedly detected not in tuber parenchyma but in the phloem of sink tubers. Immunolocalization demonstrated that StSUT1 protein was present only in sieve elements of sink tubers, cells normally involved in export of Suc from the phloem to supply developing tubers, raising the question of the role of SUT1 in tubers. SUT1 expression was inhibited by antisense in transgenic potato plants using a class I patatin promoter B33, which is primarily expressed in the phloem of developing tubers. Reduced SUT1 expression in tubers did not affect aboveground organs but led to reduced fresh weight accumulation during early stages of tuber development, indicating that in this phase SUT1 plays an important role for sugar transport. Changes in Suc- and starch-modifying enzyme activities and metabolite profiles are consistent with the developmental switch in unloading mechanisms. Altogether, the findings may suggest a role of SUT1 in retrieval of Suc from the apoplasm, thereby regulating the osmotic potential in the extracellular space, or a direct role in phloem unloading acting as a phloem exporter transferring Suc from the sieve elements into the apoplasm.

1 This work was supported by the Deutsche Forschungsgemeinschaft (grant no. SFB 446 to W.B.F.).

2 Present address: Deutsches Zentrum für Luft- und Raumfahrt e.V., D-51170 Köln, Germany.

[w] The online version of this article contains Web-only data. The supplemental material is available at www.plantphysiol.org.

* Corresponding author; e-mail christina.kuehn{at}zmbp.uni-tuebingen.de; fax 49-7071-293287.

© 2003 American Society of Plant Biologists


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