|
Plant Physiol, January 2003, Vol. 131, pp. 147-154
Extracellular ATP Inhibits Root Gravitropism at Concentrations
That Inhibit Polar Auxin Transport1
Wenqiang
Tang,
Shari R.
Brady,
Yu
Sun,
Gloria K.
Muday, and
Stanley J.
Roux*
Section of Molecular Cell and Developmental Biology, University of
Texas, Austin, Texas 78712 (W.T., Y.S., S.J.R.); and Department of
Biology, Wake Forest University, Winston-Salem, North Carolina 27109 (S.R.B., G.K.M.)
Raising the level of extracellular ATP to mM
concentrations similar to those found inside cells can block
gravitropism of Arabidopsis roots. When plants are grown in Murashige
and Skoog medium supplied with 1 mM ATP, their roots
grow horizontally instead of growing straight down. Medium with 2 mM ATP induces root curling, and 3 mM ATP
stimulates lateral root growth. When plants are transferred to medium
containing exogenous ATP, the gravity response is reduced or in some
cases completely blocked by ATP. Equivalent concentrations of ADP or
inorganic phosphate have slight but usually statistically insignificant
effects, suggesting the specificity of ATP in these responses. The ATP
effects may be attributable to the disturbance of auxin distribution in
roots by exogenously applied ATP, because extracellular ATP can alter
the pattern of auxin-induced gene expression in DR5- -glucuronidase
transgenic plants and increase the response sensitivity of plant roots
to exogenously added auxin. The presence of extracellular ATP also
decreases basipetal auxin transport in a dose-dependent fashion in both
maize (Zea mays) and Arabidopsis roots and increases the
retention of [3H]indole-3-acetic acid in root tips of
maize. Taken together, these results suggest that the inhibitory
effects of extracellular ATP on auxin distribution may happen at the
level of auxin export. The potential role of the trans-plasma membrane
ATP gradient in auxin export and plant root gravitropism is discussed.
1
This work was supported by the National
Aeronautics and Space Administration (grant no. NAG2-1347) and by the
National Science Foundation (grant no. IBN-0080363 to S.J.R.).
*
Corresponding author; e-mail sroux{at}uts.cc.utexas.edu; fax
512-232-3402.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
S. A. Reichler, J. Torres, A. L. Rivera, V. A. Cintolesi, G. Clark, and S. J. Roux
Intersection of two signalling pathways: extracellular nucleotides regulate pollen germination and pollen tube growth via nitric oxide
J. Exp. Bot.,
May 1, 2009;
60(7):
2129 - 2138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-J. Wu and J.-Y. Wu
Extracellular ATP-induced NO production and its dependence on membrane Ca2+ flux in Salvia miltiorrhiza hairy roots
J. Exp. Bot.,
October 1, 2008;
59(14):
4007 - 4016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Riewe, L. Grosman, A. R. Fernie, H. Zauber, C. Wucke, and P. Geigenberger
A Cell Wall-Bound Adenosine Nucleosidase is Involved in the Salvage of Extracellular ATP in Solanum tuberosum
Plant Cell Physiol.,
October 1, 2008;
49(10):
1572 - 1579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Foresi, A. M. Laxalt, C. V. Tonon, C. A. Casalongue, and L. Lamattina
Extracellular ATP Induces Nitric Oxide Production in Tomato Cell Suspensions
Plant Physiology,
November 1, 2007;
145(3):
589 - 592.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wu, I. Steinebrunner, Y. Sun, T. Butterfield, J. Torres, D. Arnold, A. Gonzalez, F. Jacob, S. Reichler, and S. J. Roux
Apyrases (Nucleoside Triphosphate-Diphosphohydrolases) Play a Key Role in Growth Control in Arabidopsis
Plant Physiology,
June 1, 2007;
144(2):
961 - 975.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Kim, M. Sivaguru, and G. Stacey
Extracellular ATP in Plants. Visualization, Localization, and Analysis of Physiological Significance in Growth and Signaling
Plant Physiology,
November 1, 2006;
142(3):
984 - 992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Song, I. Steinebrunner, X. Wang, S. C. Stout, and S. J. Roux
Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis
Plant Physiology,
April 1, 2006;
140(4):
1222 - 1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, J. Chen, Z. Wang, C. Yang, T. Sasaki, Y. Yamamoto, H. Matsumoto, and X. Yan
Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation
J. Exp. Bot.,
March 1, 2006;
57(6):
1353 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chivasa, B. K. Ndimba, W. J. Simon, K. Lindsey, and A. R. Slabas
Extracellular ATP Functions as an Endogenous External Metabolite Regulating Plant Cell Viability
PLANT CELL,
November 1, 2005;
17(11):
3019 - 3034.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. B. McAlvin and G. Stacey
Transgenic Expression of the Soybean Apyrase in Lotus japonicus Enhances Nodulation
Plant Physiology,
April 1, 2005;
137(4):
1456 - 1462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Jeter, W. Tang, E. Henaff, T. Butterfield, and S. J. Roux
Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis
PLANT CELL,
October 1, 2004;
16(10):
2652 - 2664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Boonsirichai, J. C. Sedbrook, R. Chen, S. Gilroy, and P. H. Masson
ALTERED RESPONSE TO GRAVITY Is a Peripheral Membrane Protein That Modulates Gravity-Induced Cytoplasmic Alkalinization and Lateral Auxin Transport in Plant Statocytes
PLANT CELL,
November 1, 2003;
15(11):
2612 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Demidchik, C. Nichols, M. Oliynyk, A. Dark, B. J. Glover, and J. M. Davies
Is ATP a Signaling Agent in Plants?
Plant Physiology,
October 1, 2003;
133(2):
456 - 461.
[Full Text]
[PDF]
|
|
|
|
|
