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Plant Physiol, January 2003, Vol. 131, pp. 237-244
Metabolic Origin of Carbon Isotope Composition of Leaf
Dark-Respired CO2 in French Bean1
Guillaume
Tcherkez,*
Salvador
Nogués,
Jean
Bleton,
Gabriel
Cornic,
Franz
Badeck, and
Jaleh
Ghashghaie
Laboratoire d'Écophysiologie Végétale,
Unité Propre de Recherche et d'Enseignement Supérieur
Associé 8079, Bâtiment 362, Université Paris
XI, 91405 Orsay, France (G.T., S.N., G.C., F.B., J.G.); and Laboratorie
d'Etudes des Techniques et Instruments d'Analyse Moléculaire,
Institut Universitaire de Technologie d'Orsay, Boite Postale 127, Plateau du Moulon, 91403 Orsay cedex, France (J.B.)
The carbon isotope composition ( 13C) of
CO2 produced in darkness by intact French bean
(Phaseolus vulgaris) leaves was investigated for
different leaf temperatures and during dark periods of increasing length. The 13C of CO2 linearly decreased
when temperature increased, from  19 at 10°C to 24 at
35°C. It also progressively decreased from 21 to 30 when
leaves were maintained in continuous darkness for several days. Under
normal conditions (temperature not exceeding 30°C and normal dark
period), the evolved CO2 was enriched in 13C
compared with carbohydrates, the most 13C-enriched
metabolites. However, at the end of a long dark period (carbohydrate
starvation), CO2 was depleted in 13C even when
compared with the composition of total organic matter. In the two types
of experiment, the variations of 13C were linearly
related to those of the respiratory quotient. This strongly suggests
that the variation of 13C is the direct consequence of a
substrate switch that may occur to feed respiration; carbohydrate
oxidation producing 13C-enriched CO2 and
 -oxidation of fatty acids producing 13C-depleted
CO2 when compared with total organic matter (27.5). These results are consistent with the assumption that the
13C of dark respired CO2 is determined by
the relative contributions of the two major decarboxylation processes
that occur in darkness: pyruvate dehydrogenase activity and the Krebs cycle.
1
This work was supported by the European Research
Training Network for Ecophysiology in Closing Terrestrial Carbon Budget
(contract no. HPRN-CT-1999-00059).
*
Corresponding author; e-mail
guillaume.tcherkez{at}ese.u-psud.fr; fax 33-1-69157238.
© 2003 American Society of Plant Biologists
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