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Plant Physiol, January 2003, Vol. 131, pp. 237-244

Metabolic Origin of Carbon Isotope Composition of Leaf Dark-Respired CO2 in French Bean1

Guillaume Tcherkez,* Salvador Nogués, Jean Bleton, Gabriel Cornic, Franz Badeck, and Jaleh Ghashghaie

Laboratoire d'Écophysiologie Végétale, Unité Propre de Recherche et d'Enseignement Supérieur Associé 8079, Bâtiment 362, Université Paris XI, 91405 Orsay, France (G.T., S.N., G.C., F.B., J.G.); and Laboratorie d'Etudes des Techniques et Instruments d'Analyse Moléculaire, Institut Universitaire de Technologie d'Orsay, Boite Postale 127, Plateau du Moulon, 91403 Orsay cedex, France (J.B.)

The carbon isotope composition (delta 13C) of CO2 produced in darkness by intact French bean (Phaseolus vulgaris) leaves was investigated for different leaf temperatures and during dark periods of increasing length. The delta 13C of CO2 linearly decreased when temperature increased, from -19per thousand at 10°C to -24per thousand at 35°C. It also progressively decreased from -21per thousand to -30per thousand when leaves were maintained in continuous darkness for several days. Under normal conditions (temperature not exceeding 30°C and normal dark period), the evolved CO2 was enriched in 13C compared with carbohydrates, the most 13C-enriched metabolites. However, at the end of a long dark period (carbohydrate starvation), CO2 was depleted in 13C even when compared with the composition of total organic matter. In the two types of experiment, the variations of delta 13C were linearly related to those of the respiratory quotient. This strongly suggests that the variation of delta 13C is the direct consequence of a substrate switch that may occur to feed respiration; carbohydrate oxidation producing 13C-enriched CO2 and beta -oxidation of fatty acids producing 13C-depleted CO2 when compared with total organic matter (-27.5per thousand ). These results are consistent with the assumption that the delta 13C of dark respired CO2 is determined by the relative contributions of the two major decarboxylation processes that occur in darkness: pyruvate dehydrogenase activity and the Krebs cycle.

1 This work was supported by the European Research Training Network for Ecophysiology in Closing Terrestrial Carbon Budget (contract no. HPRN-CT-1999-00059).

* Corresponding author; e-mail guillaume.tcherkez{at}ese.u-psud.fr; fax 33-1-69157238.

© 2003 American Society of Plant Biologists


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