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Plant Physiol, January 2003, Vol. 131, pp. 245-253
RNA Target Sequences Promote Spreading of RNA
Silencing1
Helena
Van Houdt,
Annick
Bleys, and
Anna
Depicker*
Department of Plant Systems Biology, Flanders Interuniversity
Institute for Biotechnology, Ghent University, Karel Lodewijk
Ledeganckstraat 35, B-9000 Ghent, Belgium
It is generally recognized that a silencing-inducing locus
can efficiently reduce the expression of genes that give rise to transcripts partially homologous to those produced by the
silencing-inducing locus (primary targets). Interestingly, the
expression of genes that produce transcripts without homology to the
silencing-inducing locus (secondary targets) can also be decreased
dramatically via transitive RNA silencing. This phenomenon requires
primary target RNAs that contain sequences homologous to secondary
target RNAs. Sequences upstream from the region homologous to the
silencing inducer in the primary target transcripts give rise to
approximately 22-nucleotide small RNAs, coinciding with the region
homologous to the secondary target. The presence of these small RNAs
corresponds with reduced expression of the secondary target whose
transcripts are not homologous to the silencing inducer. The data
suggest that in transgenic plants, targets of RNA silencing are
involved in the expansion of the pool of functional small interfering
RNAs. Furthermore, methylation of target genes in sequences without homology to the initial silencing inducer indicates not only that RNA
silencing can expand across target RNAs but also that methylation can
spread along target genes.
1
H.V.H. received a postdoctoral fellowship from
the Instituut voor de aanmoediging van Innovatie door Wetenschap en
Technologie in Vlaanderen.
*
Corresponding author; e-mail anna.depicker{at}rug.ac.be; fax
32-9-264-5349.
© 2003 American Society of Plant Biologists
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