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Plant Physiol, January 2003, Vol. 131, pp. 309-316
The binding of Maize DHN1 to Lipid Vesicles. Gain of Structure
and Lipid Specificity1
Myong-Chul
Koag,
Raymond D.
Fenton,
Stephan
Wilkens, and
Timothy J.
Close*
Graduate Program in Biochemistry and Molecular Biology (M.-C.K.,
S.W., T.J.C.) and Departments of Botany and Plant Sciences (M.-C.K.,
R.D.F., T.J.C.) and Biochemistry (S.W.), University of California,
Riverside, California 92521-0124
Dehydrins (DHNs; late embryogenesis abundant D-11) are a
family of plant proteins induced in response to abiotic stresses such
as drought, low temperature, and salinity or during the late stages of
embryogenesis. Spectral and thermal properties of these proteins in
purified form suggest that they are "intrinsically unstructured."
However, DHNs contain at least one copy of a consensus 15-amino acid
sequence, the "K segment," which resembles a class A2 amphipathic
 -helical, lipid-binding domain found in other proteins such as
apolipoproteins and -synuclein. The presence of the K segment raises
the question of whether DHNs bind lipids, bilayers, or phospholipid
vesicles. Here, we show that maize (Zea mays) DHN DHN1
can bind to lipid vesicles that contain acidic phospholipids. We also
observe that DHN1 binds more favorably to vesicles of smaller diameter
than to larger vesicles, and that the association of DHN1 with vesicles
results in an apparent increase of -helicity of the protein.
Therefore, DHNs, and presumably somewhat similar plant stress proteins
in the late embryogenesis abundant and cold-regulated classes may
undergo function-related conformational changes at the water/membrane
interface, perhaps related to the stabilization of vesicles or other
endomembrane structures under stress conditions.
1
This work was supported by the National Science
Foundation (grant no. IBN 92-05269), by the University of
California (Biotechnology Research and Education grant no. 97-15), and
by the California Agricultural Experiment Station Funds (Hatch grant
no. 5306-H).
*
Corresponding author; e-mail timothy.close{at}ucr.edu; fax
909-787-4437.
© 2003 American Society of Plant Biologists
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