Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Atanassova, R.
Right arrow Articles by Delrot, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atanassova, R.
Right arrow Articles by Delrot, S.
Agricola
Right arrow Articles by Atanassova, R.
Right arrow Articles by Delrot, S.

Plant Physiol, January 2003, Vol. 131, pp. 326-334

Sugar-Regulated Expression of a Putative Hexose Transport Gene in Grape1

Rossitza Atanassova,* Marina Leterrier, Cécile Gaillard, Alice Agasse, Emeric Sagot, Pierre Coutos-Thévenot, and Serge Delrot

Unité Mixte de Recherche-Centre National de la Recherche Scientifique 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaires Végétales, Bâtiment Botanique, Unité Fondamentale de Recherche Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers cedex, France

Different lengths of the promoter of grape (Vitis vinifera) VvHT1 (Hexose Transporter 1) gene, which encodes a putative hexose transporter expressed during the ripening of grape, have been transcriptionally fused to the beta -glucuronidase reporter gene. In transgenic tobacco (Nicotiana tabacum) transformed with these constructs, VvHT1 promoters were clearly responsible for the sink organ preferential expression. The potential sugar effectors of VvHT1 promoter were studied in tobacco cv Bright-Yellow 2 cells transformed with chimeric constructs. Glucose (56 mM), sucrose (Suc; 58 mM), and the non-transported Suc isomer palatinose doubled the beta -glucuronidase activity conferred by the VvHT1 promoter, whereas fructose did not affect it. These effects were the strongest with the 2.4-kb promoter, which contains all putative sugar-responsive elements (activating and repressing), but they were also significant with the 0.3-kb promoter, which contains only activating sugar boxes. The induction of VvHT1 expression by both Suc and palatinose was confirmed in the homologous grape berry cell culture. The data provide the first example of a putative sugar transporter, which is induced by both glucose and Suc in higher plants. Although induction of VvHT1 expression by Suc does not require transport, the presence of glucosyl moiety is necessary for Suc sensing. These results provide new insights into sugar sensing and signaling in plants.

1 This work was supported by the Conseil Régional Poitou-Charentes, France (to M.L.).

* Corresponding author; e-mail Rossitza.Atanassova{at}univ-poitiers.fr; fax 33-0-5-49-45-41-86.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R.-C. Fan, C.-C. Peng, Y.-H. Xu, X.-F. Wang, Y. Li, Y. Shang, S.-Y. Du, R. Zhao, X.-Y. Zhang, L.-Y. Zhang, et al.
Apple Sucrose Transporter SUT1 and Sorbitol Transporter SOT6 Interact with Cytochrome b5 to Regulate Their Affinity for Substrate Sugars
Plant Physiology, August 1, 2009; 150(4): 1880 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. K. Proels and T. Roitsch
Extracellular invertase LIN6 of tomato: a pivotal enzyme for integration of metabolic, hormonal, and stress signals is regulated by a diurnal rhythm
J. Exp. Bot., April 1, 2009; 60(6): 1555 - 1567.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. T. Matus, R. Loyola, A. Vega, A. Pena-Neira, E. Bordeu, P. Arce-Johnson, and J. A. Alcalde
Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera
J. Exp. Bot., March 1, 2009; 60(3): 853 - 867.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Zhou, K. Chan, T. L. Wang, C. L. Hedley, C. E. Offler, and J. W. Patrick
Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons
J. Exp. Bot., January 1, 2009; 60(1): 71 - 85.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Lebon, G. Wojnarowiez, B. Holzapfel, F. Fontaine, N. Vaillant-Gaveau, and C. Clement
Sugars and flowering in the grapevine (Vitis vinifera L.)
J. Exp. Bot., July 1, 2008; 59(10): 2565 - 2578.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Lejay, J. Wirth, M. Pervent, J. M.-F. Cross, P. Tillard, and A. Gojon
Oxidative Pentose Phosphate Pathway-Dependent Sugar Sensing as a Mechanism for Regulation of Root Ion Transporters by Photosynthesis
Plant Physiology, April 1, 2008; 146(4): 2036 - 2053.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. N. Oliver, E. S. Dennis, and R. Dolferus
ABA Regulates Apoplastic Sugar Transport and is a Potential Signal for Cold-Induced Pollen Sterility in Rice
Plant Cell Physiol., September 1, 2007; 48(9): 1319 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Hanana, O. Cagnac, T. Yamaguchi, S. Hamdi, A. Ghorbel, and E. Blumwald
A Grape Berry (Vitis vinifera L.) Cation/Proton Antiporter is Associated with Berry Ripening
Plant Cell Physiol., June 1, 2007; 48(6): 804 - 811.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology, August 1, 2006; 141(4): 1563 - 1577.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Goes da Silva, A. Iandolino, F. Al-Kayal, M. C. Bohlmann, M. A. Cushman, H. Lim, A. Ergul, R. Figueroa, E. K. Kabuloglu, C. Osborne, et al.
Characterizing the Grape Transcriptome. Analysis of Expressed Sequence Tags from Multiple Vitis Species and Development of a Compendium of Gene Expression during Berry Development
Plant Physiology, October 1, 2005; 139(2): 574 - 597.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot., May 1, 2005; 56(415): 1409 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Wiese, N. Elzinga, B. Wobbes, and S. Smeekens
A Conserved Upstream Open Reading Frame Mediates Sucrose-Induced Repression of Translation
PLANT CELL, July 1, 2004; 16(7): 1717 - 1729.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Maurel, S. Sakr, F. Gerbe, A. Guilliot, M. Bonhomme, R. Rageau, and G. Petel
Sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree buds
J. Exp. Bot., April 1, 2004; 55(398): 879 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. Frankel, E. Hasson, N. D. Iusem, and M. S. Rossi
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats
Mol. Biol. Evol., December 1, 2003; 20(12): 1955 - 1962.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. Cakir, A. Agasse, C. Gaillard, A. Saumonneau, S. Delrot, and R. Atanassova
A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling
PLANT CELL, September 1, 2003; 15(9): 2165 - 2180.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists