First published online January 23, 2003; 10.1104/pp.015479
Plant Physiol, February 2003, Vol. 131, pp. 472-481
Identification of Mutants of Arabidopsis Defective in Acclimation
of Photosynthesis to the Light Environment1
Robin G.
Walters,2*
Freya
Shephard,
Jennifer J.M.
Rogers,
Stephen A.
Rolfe, and
Peter
Horton
Department of Molecular Biology and Biotechnology (R.G.W.,
F.S., J.J.M.R., P.H.), and Department of Animal and Plant Sciences
(S.A.R.), University of Sheffield, Western Bank, Sheffield S10 2TN,
United Kingdom
In common with many other higher plant species, Arabidopsis
undergoes photosynthetic acclimation, altering the composition of the
photosynthetic apparatus in response to fluctuations in its growth
environment. The changes in photosynthetic function that result from
acclimation can be detected in a noninvasive manner by monitoring
chlorophyll (Chl) fluorescence. This technique has been used to develop
a screen that enables the rapid identification of plants defective at
ACCLIMATION OF PHOTOSYNTHESIS TO THE ENVIRONMENT (APE) loci. The application of this screen to a
population of T-DNA-transformed Arabidopsis has successfully led to the
identification of a number of mutant lines with altered Chl
fluorescence characteristics. Analysis of photosynthesis and pigment
composition in leaves from three such mutants showed that they had
altered acclimation responses to the growth light environment, each
having a distinct acclimation-defective phenotype, demonstrating that
screening for mutants using Chl fluorescence is a viable strategy for
the investigation of acclimation. Sequencing of the genomic DNA
flanking the T-DNA elements showed that in the ape1
mutant, a gene was disrupted that encodes a protein of unknown function
but that appears to be specific to photosynthetic organisms, whereas
the ape2 mutant carries an insertion in the region of
the TPT gene encoding the chloroplast inner envelope triose phosphate/phosphate translocator.
1
This work was supported by the U.K.
Biotechnology and Biological Sciences Research Council (grant no.
50/P08723).
2
Present address: Department of Plant Sciences,
University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
*
Corresponding author; e-mail robin.walters{at}plants.ox.ac.uk;
fax 44-1865-275074.
© 2003 American Society of Plant Biologists
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