First published online January 9, 2003; 10.1104/pp.014555
Plant Physiol, February 2003, Vol. 131, pp. 547-557
AtCSLA7, a Cellulose Synthase-Like Putative Glycosyltransferase,
Is Important for Pollen Tube Growth and Embryogenesis in
Arabidopsis1
Florence
Goubet,
Audrey
Misrahi,
Soon Ki
Park,
Zhinong
Zhang,
David
Twell, and
Paul
Dupree*
Department of Biochemistry, University of Cambridge,
Building O, Downing Site, Cambridge CB2 1QW, United Kingdom (F.G.,
A.M., Z.Z., P.D.); and Department of Biology, University of Leicester,
University Road, Leicester LE1 7RH, United Kingdom (S.K.P.,
D.T.)
The cellulose synthase-like proteins are a large
family of proteins in plants thought to be processive polysaccharide
 -glycosyltransferases. We have characterized an Arabidopsis mutant
with a transposon insertion in the gene encoding AtCSLA7 of the CSLA
subfamily. Analysis of the transmission efficiency of the insertion
indicated that AtCSLA7 is important for pollen tube growth. Moreover,
the homozygous insertion was embryo lethal. A detailed analysis of seed
developmental progression revealed that mutant embryos developed more
slowly than wild-type siblings. The mutant embryos also showed abnormal
cell patterning and they arrested at a globular stage. The defective
embryonic development was associated with reduced proliferation and
failed cellularization of the endosperm. AtCSLA7 is
widely expressed, and is likely to be required for synthesis of a cell
wall polysaccharide found throughout the plant. Our results suggest
that this polysaccharide is essential for cell wall structure or for
signaling during plant embryo development.
1
This work was supported by the Biotechnology and
Biological Sciences Research Council and by DuPont (UK).
*
Corresponding author; e-mail p.dupree{at}bioc.cam.ac.uk; fax
44-1223 333345.
© 2003 American Society of Plant Biologists
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