First published online January 23, 2003; 10.1104/pp.014431
Plant Physiol, February 2003, Vol. 131, pp. 603-609
Functional Divergence of a Syntenic Invertase Gene Family in
Tomato, Potato, and Arabidopsis1
Eyal
Fridman and
Dani
Zamir*
Department of Molecular, Cellular and Developmental Biology, The
University of Michigan, Ann Arbor, Michigan 48109-1048 (E.F.); and
Department of Field and Vegetable Crops and The Otto Warburg Center for
Biotechnology, Faculty of Agriculture, The Hebrew University of
Jerusalem, P.O. Box 12, Rehovot 76100, Israel (D.Z.)
Comparative analysis of complex developmental pathways
depends on our ability to resolve the function of members of gene
families across taxonomic groups. LIN5, which belongs to
a small gene family of apoplastic invertases in tomato
(Lycopersicon esculentum), is a quantitative trait locus
that modifies fruit sugar composition. We have compared the genomic
organization and expression of this gene family in the two distantly
related species: tomato and Arabidopsis. Invertase family members
reside on segmental duplications in the near-colinear genomes of tomato
and potato (Solanum tuberosum). These chromosomal
segments are syntenically duplicated in the model plant Arabidopsis. On
the basis of phylogenetic analysis of genes in the microsyntenic
region, we conclude that these segmental duplications arose
independently after the separation of the tomato/potato clade from
Arabidopsis. Rapid regulatory divergence is characteristic of the
invertase family. Interestingly, although the processes of gene
duplication and specialization of expression occurred separately in the
two species, synteny-based orthologs from both clades acquired similar
organ-specific expression. This similar expression pattern of the genes
is evidence of comparable evolutionary constraints (parallel evolution)
rather than of functional orthology. The observation that functional
orthology cannot be identified through analysis of expression
similarity highlights the caution that needs to be exercised in
extrapolating developmental networks from a model organism.
1
This work was supported by the United
States-Israel Binational Science Foundation.
*
Corresponding author; e-mail zamir{at}agri.huji.ac.il; fax
972-8-9489092.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
Y. Jin, D.-A. Ni, and Y.-L. Ruan
Posttranslational Elevation of Cell Wall Invertase Activity by Silencing Its Inhibitor in Tomato Delays Leaf Senescence and Increases Seed Weight and Fruit Hexose Level
PLANT CELL,
July 1, 2009;
21(7):
2072 - 2089.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. Zanor, S. Osorio, A. Nunes-Nesi, F. Carrari, M. Lohse, B. Usadel, C. Kuhn, W. Bleiss, P. Giavalisco, L. Willmitzer, et al.
RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility
Plant Physiology,
July 1, 2009;
150(3):
1204 - 1218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Proels and T. Roitsch
Extracellular invertase LIN6 of tomato: a pivotal enzyme for integration of metabolic, hormonal, and stress signals is regulated by a diurnal rhythm
J. Exp. Bot.,
April 1, 2009;
60(6):
1555 - 1567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Bertin, M. Causse, B. Brunel, D. Tricon, and M. Genard
Identification of growth processes involved in QTLs for tomato fruit size and composition
J. Exp. Bot.,
January 1, 2009;
60(1):
237 - 248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kocal, U. Sonnewald, and S. Sonnewald
Cell Wall-Bound Invertase Limits Sucrose Export and Is Involved in Symptom Development and Inhibition of Photosynthesis during Compatible Interaction between Tomato and Xanthomonas campestris pv vesicatoria
Plant Physiology,
November 1, 2008;
148(3):
1523 - 1536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Essmann, I. Schmitz-Thom, H. Schon, S. Sonnewald, E. Weis, and J. Scharte
RNA Interference-Mediated Repression of Cell Wall Invertase Impairs Defense in Source Leaves of Tobacco
Plant Physiology,
July 1, 2008;
147(3):
1288 - 1299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schaarschmidt, T. Roitsch, and B. Hause
Arbuscular mycorrhiza induces gene expression of the apoplastic invertase LIN6 in tomato (Lycopersicon esculentum) roots
J. Exp. Bot.,
December 1, 2006;
57(15):
4015 - 4023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot.,
June 1, 2006;
57(9):
1883 - 1897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Quilliam, P. J. Swarbrick, J. D. Scholes, and S. A. Rolfe
Imaging photosynthesis in wounded leaves of Arabidopsis thaliana
J. Exp. Bot.,
January 1, 2006;
57(1):
55 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, J. Strahwald, H.-R. Hofferbert, J. Lubeck, E. Tacke, H. Junghans, J. Wunder, and C. Gebhardt
DNA Variation at the Invertase Locus invGE/GF Is Associated With Tuber Quality Traits in Populations of Potato Breeding Clones
Genetics,
June 1, 2005;
170(2):
813 - 821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Baxter, F. Carrari, A. Bauke, S. Overy, S. A. Hill, P. W. Quick, A. R. Fernie, and L. J. Sweetlove
Fruit Carbohydrate Metabolism in an Introgression Line of Tomato with Increased Fruit Soluble Solids
Plant Cell Physiol.,
March 1, 2005;
46(3):
425 - 437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Overy, H. J. Walker, S. Malone, T. P. Howard, C. J. Baxter, L. J. Sweetlove, S. A. Hill, and W. P. Quick
Application of metabolite profiling to the identification of traits in a population of tomato introgression lines
J. Exp. Bot.,
January 1, 2005;
56(410):
287 - 296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Fridman, F. Carrari, Y.-S. Liu, A. R. Fernie, and D. Zamir
Zooming In on a Quantitative Trait for Tomato Yield Using Interspecific Introgressions
Science,
September 17, 2004;
305(5691):
1786 - 1789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
