Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 9, 2003; 10.1104/pp.011445

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
131/2/676    most recent
pp.011445v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (60)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carden, D. E.
Right arrow Articles by Miller, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carden, D. E.
Right arrow Articles by Miller, A. J.
Agricola
Right arrow Articles by Carden, D. E.
Right arrow Articles by Miller, A. J.

Plant Physiol, February 2003, Vol. 131, pp. 676-683

Single-Cell Measurements of the Contributions of Cytosolic Na+ and K+ to Salt Tolerance1

David E. Carden,2 David J. Walker,3 Timothy J. Flowers, and Anthony J. Miller*

Agriculture and Environment Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (D.E.C., D.J.W., A.J.M.); and School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG, United Kingdom (T.J.F.)

Ion concentrations in the roots of two barley (Hordeum vulgare) varieties that differed in NaCl tolerance were compared after exposure to NaCl. Triple-barreled H+-, K+-, and Na+-selective microelectrodes were used to measure cytosolic activities of the three ions after 5 and 8 d of NaCl stress. In both varieties of barley, it was only possible to record successfully from root cortical cells because the epidermal cells appeared to be damaged. The data show that from the 1st d of full NaCl stress, there were differences in the way in which the two varieties responded. At 5 d, the tolerant variety maintained a 10-fold lower cytosolic Na+ than the more sensitive variety, although by 8 d the two varieties were not significantly different. At this time, the more tolerant variety was better at maintaining root cytosolic K+ in the high-NaCl background than was the more sensitive variety. In contrast to earlier work on K+-starved barley (Walker et al., 1996), there was no acidification of the cytosol associated with the decreased cytosolic K+ activity during NaCl stress. These single-cell measurements of cytosolic and vacuolar ion activities allow calculation of thermodynamic gradients that can be used to reveal (or predict) the type of active transporters at both the plasma membrane and tonoplast.

1 This work and Rothamsted Research are supported by the Biotechnology and Biological Sciences Research Council (UK; studentship award to D.E.C.).

2 Present address: Università degli Studi di Padova, Dipartimento di Biotecnologie Agrarie, Agripolis, Via Romea 16, 35020 Legnaro, Italy.

3 Present address: Department of Environmental Resources, Centro de Edafología y Biología Aplicada del Segura, Apartado 4195, 30080 Murcia, Spain.

* Corresponding author; e-mail tony.miller{at}bbsrc.ac.uk; fax 44-1582-763010.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
B. Benito, B. Garciadeblas, J. Perez-Martin, and A. Rodriguez-Navarro
Growth at High pH and Sodium and Potassium Tolerance in Media above the Cytoplasmic pH Depend on ENA ATPases in Ustilago maydis
Eukaryot. Cell, June 1, 2009; 8(6): 821 - 829.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. A. Cuin, S. A. Betts, R. Chalmandrier, and S. Shabala
A root's ability to retain K+ correlates with salt tolerance in wheat
J. Exp. Bot., July 1, 2008; 59(10): 2697 - 2706.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. J. Kronzucker, M. W. Szczerba, L. M. Schulze, and D. T. Britto
Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.)
J. Exp. Bot., July 1, 2008; 59(10): 2793 - 2801.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Chen, T. A. Cuin, M. Zhou, A. Twomey, B. P. Naidu, and S. Shabala
Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance
J. Exp. Bot., December 1, 2007; 58(15-16): 4245 - 4255.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Chen, I. I. Pottosin, T. A. Cuin, A. T. Fuglsang, M. Tester, D. Jha, I. Zepeda-Jazo, M. Zhou, M. G. Palmgren, I. A. Newman, et al.
Root Plasma Membrane Transporters Controlling K+/Na+ Homeostasis in Salt-Stressed Barley
Plant Physiology, December 1, 2007; 145(4): 1714 - 1725.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Zhao, C.-P. Song, J. He, and H. Zhu
Polyamines Improve K+/Na+ Homeostasis in Barley Seedlings by Regulating Root Ion Channel Activities
Plant Physiology, November 1, 2007; 145(3): 1061 - 1072.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Shabala, V. Demidchik, L. Shabala, T. A. Cuin, S. J. Smith, A. J. Miller, J. M. Davies, and I. A. Newman
Extracellular Ca2+ Ameliorates NaCl-Induced K+ Loss from Arabidopsis Root and Leaf Cells by Controlling Plasma Membrane K+-Permeable Channels
Plant Physiology, August 1, 2006; 141(4): 1653 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. W. Szczerba, D. T. Britto, and H. J. Kronzucker
Rapid, Futile K+ Cycling and Pool-Size Dynamics Define Low-Affinity Potassium Transport in Barley
Plant Physiology, August 1, 2006; 141(4): 1494 - 1507.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Rodriguez-Navarro and F. Rubio
High-affinity potassium and sodium transport systems in plants
J. Exp. Bot., March 1, 2006; 57(5): 1149 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. M. Pardo, B. Cubero, E. O. Leidi, and F. J. Quintero
Alkali cation exchangers: roles in cellular homeostasis and stress tolerance
J. Exp. Bot., March 1, 2006; 57(5): 1181 - 1199.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Md. A. Kader and S. Lindberg
Uptake of sodium in protoplasts of salt-sensitive and salt-tolerant cultivars of rice, Oryza sativa L. determined by the fluorescent dye SBFI
J. Exp. Bot., December 1, 2005; 56(422): 3149 - 3158.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. A. Cuin and S. Shabala
Exogenously Supplied Compatible Solutes Rapidly Ameliorate NaCl-induced Potassium Efflux from Barley Roots
Plant Cell Physiol., December 1, 2005; 46(12): 1924 - 1933.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Rubio, A. Linares-Rueda, M. J. Garcia-Sanchez, and J. A. Fernandez
Physiological evidence for a sodium-dependent high-affinity phosphate and nitrate transport at the plasma membrane of leaf and root cells of Zostera marina L.
J. Exp. Bot., February 1, 2005; 56(412): 613 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Qi and E. P. Spalding
Protection of Plasma Membrane K+ Transport by the Salt Overly Sensitive1 Na+-H+ Antiporter during Salinity Stress
Plant Physiology, September 1, 2004; 136(1): 2548 - 2555.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. J. Halperin and J. P. Lynch
Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: in vivo measurements using the fluorescent dyes SBFI and PBFI
J. Exp. Bot., September 1, 2003; 54(390): 2035 - 2043.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists