First published online January 9, 2003; 10.1104/pp.012682
Plant Physiol, February 2003, Vol. 131, pp. 697-706
Zonal Changes in Ascorbate and Hydrogen Peroxide Contents,
Peroxidase, and Ascorbate-Related Enzyme Activities in Onion
Roots1
María
del Carmen
Córdoba-Pedregosa,
Francisco
Córdoba,
José Manuel
Villalba, and
José Antonio
González-Reyes*
Departamento de Biología Celular, Fisiología e
Inmunología, University of Córdoba, 14014 Córdoba,
Spain (M.d.C.C.-P., J.M.V., J.A.G.-R.); and Departamento de
Biología Ambiental y Salud Pública, University of Huelva,
21014 Huelva, Spain (F.C.)
Onion (Allium cepa) roots growing
hydroponically show differential zonal values for intra- (symplastic)
and extra- (apoplastic) cellular ascorbate (ASC) and
dehydroascorbate (DHA) contents and for related enzyme activities. In
whole roots, ASC and DHA concentrations were higher in root apex and
meristem and gradually decreased toward the root base. Guaiacol
peroxidase, ASC peroxidase, monodehydroascorbate oxidoreductase, DHA
reductase, catalase, and glutathione reductase activities showed
differential activity patterns depending on the zone of the root and
their apoplastic or symplastic origin. An in vivo staining of
peroxidase activity also revealed a specific distribution pattern along
the root axis. Using electron microscopy, hydrogen peroxide was found
at different locations depending on the root zone but was mainly
located in cell walls from epidermal and meristematic cells and in
cells undergoing lignification. A balanced control of all of these
molecules seems to exist along the root axis and may be directly
related to the mechanisms in which the ASC system is involved, as cell
division and elongation. The role of ASC on growth and development in
relation to its presence at the different zones of the root is discussed.
1
This work was supported by the Spanish
Ministerio de Educación y Cultura (grant nos.
PB98-0329-CO2-02, 1FD97-0457-CO2-02, and BMC2002-01078) and by
the Junta de Andalucía (grant no. CVI-267 to
M.d.C.C.-P.).
*
Corresponding author; e-mail bc1gorej{at}uco.es;
fax 34-957-218634.
© 2003 American Society of Plant Biologists
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