First published online January 23, 2003; 10.1104/pp.013169
Plant Physiol, February 2003, Vol. 131, pp. 763-772
Expression of Cytosolic and Plastid Acetyl-Coenzyme A Carboxylase
Genes in Young Wheat Plants1,[w]
Jan
Podkowinski,2
Joanna
Jelenska,
Anchalee
Sirikhachornkit,
Ellen
Zuther,3
Robert
Haselkorn, and
Piotr
Gornicki*
Department of Molecular Genetics and Cell Biology, University of
Chicago, Chicago, Illinois 60637
Expression of cytosolic and plastid acetyl-coenzyme A
carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major
plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice
site for the first intron accumulates to its highest level in roots.
Cytosolic ACCase mRNA also consists of two species, one of which is
present at approximately a constant level, whereas the other
accumulates to a high level in the lower sheath section. It is likely
that different promoters are also responsible for the two forms of
cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase
mRNAs in the sheath section of the plant are similar. ACCase protein
level is significantly lower in the leaf blades, in parallel with
changes in the total ACCase mRNA level. Homoeologous ACCase genes show
the same expression patterns and similar mRNA levels, suggesting that
none of the genes was silenced or acquired new tissue specificity after polyploidization.
1
This work was supported by the Consortium for
Plant Biotechnology Research (grant), by the Monsanto Co. (grant), and
by the German Academic Exchange Service (fellowship to E.Z.).
2
Present address: Institute of Bioorganic
Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61704 Poznan, Poland.
3
Present address: Max-Planck-Institute for Molecular
Plant Physiology, Am Muhlenberg 1, 11476 Golm, Germany.
*
Corresponding author; e-mail pg13{at}midway.uchicago.edu; fax
773-702-3172.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
© 2003 American Society of Plant Biologists
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