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Plant Physiol, February 2003, Vol. 131, pp. 803-813
Polyamine Oxidase, a Hydrogen Peroxide-Producing Enzyme, Is
Up-Regulated by Light and Down-Regulated by Auxin in the Outer Tissues
of the Maize Mesocotyl1
Alessandra
Cona,
Francesco
Cenci,2
Manuela
Cervelli,
Rodolfo
Federico,
Paolo
Mariottini,
Sandra
Moreno, and
Riccardo
Angelini*
Dipartimento di Biologia, Università degli Studi "Roma
Tre," Viale Guglielmo Marconi 446, 00146 Rome, Italy
Exogenously supplied auxin (1-naphthaleneacetic acid)
inhibited light-induced activity increase of polyamine oxidase (PAO), a
hydrogen peroxide-producing enzyme, in the outer tissues of maize
(Zea mays) mesocotyl. The same phenomenon operates at
PAO protein and mRNA accumulation levels. The wall-bound to extractable PAO activity ratio was unaffected by auxin treatment, either in the
dark or after light exposure. Ethylene treatment did not affect PAO
activity, thus excluding an effect of auxin via increased ethylene
biosynthesis. The auxin polar transport inhibitors
N1-naphthylphthalamic acid or
2,3,5-triiodobenzoic acid caused a further increase of PAO expression
in outer tissues after light treatment. The small increase of PAO
expression, normally occurring in the mesocotyl epidermis during plant
development in the dark, was also inhibited by auxin, although to a
lesser extent with respect to light-exposed tissue, and was stimulated
by N1-naphthylphthalamic acid or
2,3,5-triiodobenzoic acid, thus suggesting a complex regulation of PAO
expression. Immunogold ultrastructural analysis in epidermal cells
revealed the association of PAO with the secretory pathway and the cell
walls. The presence of the enzyme in the cell walls of this tissue
greatly increased in response to light treatment. Consistent with auxin
effects on light-induced PAO expression, the hormone treatment
inhibited the increase in immunogold staining both
intraprotoplasmically and in the cell wall. These results suggest that
both light and auxin finely tune PAO expression during the
light-induced differentiation of the cell wall in the maize mesocotyl
epidermal tissues.
1
This work was supported by the Italian Ministry
for University and Research.
2
Present address: Research Center, Ospedale Pediatrico
Bambino Gesù, Università "Tor Vergata," Via di Tor
Vergata 135, Rome 00133, Italy.
*
Corresponding author; e-mail angelini{at}bio.uniroma3.it; fax
06-55-176-321.
© 2003 American Society of Plant Biologists
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