First published online January 23, 2003; 10.1104/pp.010538
Plant Physiol, February 2003, Vol. 131, pp. 824-837
In Plants, 3-O-Methylglucose Is Phosphorylated by
Hexokinase But Not Perceived as a Sugar1
Sandra
Cortès,
Marina
Gromova,
Adeline
Evrard,
Claude
Roby,*
Alain
Heyraud,
Dominique B.
Rolin,
Philippe
Raymond, and
Renaud M.
Brouquisse
Commissariat à l'Energie Atomique, Centre National de la
Recherche Scientifique, and Université Joseph Fourier,
Unité Mixte de Recherche 5019 Physiologie Cellulaire
Végétale, 17 rue des Martyrs, 38054 Grenoble cedex
9, France (S.C., M.G., A.E., C.R.); Centre National de la Recherche
Scientifique, Unité Propre de Recherche 5301 Centre de
Recherches sur les Macromolécules Végétales,
Boîte Postale 53, 38041 Grenoble cedex 9, France (A.H.);
and Institut National de la Recherche Agronomique and Université
Victor Segalen Bordeaux 2, Unité Mixte de Recherche 619 Biotechnologie et Physiologie Végétales, Boîte
Postale 81, 33883 Villenave d'Ornon cedex, France (D.B.R., P.R.,
R.M.B.)
In plants, sugars are the main respiratory substrates and
important signaling molecules in the regulation of carbon metabolism. Sugar signaling studies suggested that sugar sensing involves several
key components, among them hexokinase (HXK). Although the sensing
mechanism of HXK is unknown, several experiments support the hypothesis
that hexose phosphorylation is a determining factor. Glucose (Glc)
analogs transported into cells but not phosphorylated are frequently
used to test this hypothesis, among them 3-O-methyl-Glc (3-OMG). The aim of the present work was to investigate the effects and
fate of 3-OMG in heterotrophic plant cells. Measurements of respiration
rates, protein and metabolite contents, and protease activities and
amounts showed that 3-OMG is not a respiratory substrate and does not
contribute to biosynthesis. Proteolysis and lipolysis are induced in
3-OMG-fed maize (Zea mays L. cv DEA) roots in the same
way as in sugar-starved organs. However, contrary to the generally
accepted idea, phosphorous and carbon nuclear magnetic resonance
experiments and enzymatic assays prove that 3-OMG is phosphorylated to
3-OMG-6-phosphate, which accumulates in the cells. Insofar as plant HXK
is involved in sugar sensing, these findings are discussed on the basis
of the kinetic properties because the catalytic efficiency of HXK
isolated from maize root tips is five orders of magnitude lower for
3-OMG than for Glc and Man.
1
This work was supported by the Centre National
de la Recherche Scientifique, by the Commissariat à l'Energie
Atomique, by the Institut National de la Recherche Agronomique, by the
Université Victor Segalen in Bordeaux, by the Université
Joseph Fourier in Grenoble, in part by the EC (grant no.
BIO4-CT96-0311), and by the Ministère de l'Education
Nationale, de la Recherche, et de la Technologie (grants to S.C. and
A.E.).
*
Corresponding author; e-mail croby{at}cea.fr; fax
33-4-38-78-54-83.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
P. K. Khera, C. H. Joiner, A. Carruthers, C. J. Lindsell, E. P. Smith, R. S. Franco, Y. R. Holmes, and R. M. Cohen
Evidence for Interindividual Heterogeneity in the Glucose Gradient Across the Human Red Blood Cell Membrane and Its Relationship to Hemoglobin Glycation
Diabetes,
September 1, 2008;
57(9):
2445 - 2452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Knight, A. J.W. Thomson, and H. G. McWatters
SENSITIVE TO FREEZING6 Integrates Cellular and Environmental Inputs to the Plant Circadian Clock
Plant Physiology,
September 1, 2008;
148(1):
293 - 303.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Tholen, T. L. Pons, L. A.C.J. Voesenek, and H. Poorter
Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco
Plant Physiology,
July 1, 2007;
144(3):
1305 - 1315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Deuschle, B. Chaudhuri, S. Okumoto, I. Lager, S. Lalonde, and W. B. Frommer
Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants
PLANT CELL,
September 1, 2006;
18(9):
2314 - 2325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology,
August 1, 2006;
141(4):
1563 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Cernac, C. Andre, S. Hoffmann-Benning, and C. Benning
WRI1 Is Required for Seed Germination and Seedling Establishment
Plant Physiology,
June 1, 2006;
141(2):
745 - 757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Alonso, H. Vigeolas, P. Raymond, D. Rolin, and M. Dieuaide-Noubhani
A New Substrate Cycle in Plants. Evidence for a High Glucose-Phosphate-to-Glucose Turnover from in Vivo Steady-State and Pulse-Labeling Experiments with [13C]Glucose and [14C]Glucose
Plant Physiology,
August 1, 2005;
138(4):
2220 - 2232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Price, A. Laxmi, S. K. St. Martin, and J.-C. Jang
Global Transcription Profiling Reveals Multiple Sugar Signal Transduction Mechanisms in Arabidopsis
PLANT CELL,
August 1, 2004;
16(8):
2128 - 2150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Maurel, S. Sakr, F. Gerbe, A. Guilliot, M. Bonhomme, R. Rageau, and G. Petel
Sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree buds
J. Exp. Bot.,
April 1, 2004;
55(398):
879 - 888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Gibson
Sugar and phytohormone response pathways: navigating a signalling network
J. Exp. Bot.,
January 2, 2004;
55(395):
253 - 264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hauschild and A. von Schaewen
Differential Regulation of Glucose-6-Phosphate Dehydrogenase Isoenzyme Activities in Potato
Plant Physiology,
September 1, 2003;
133(1):
47 - 62.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
