First published online February 13, 2003; 10.1104/pp.102.016071
Plant Physiol, March 2003, Vol. 131, pp. 1009-1017
The Sym35 Gene Required for Root Nodule Development
in Pea Is an Ortholog of Nin from Lotus
japonicus1
Alexey Y.
Borisov,
Lene H.
Madsen,
Viktor E.
Tsyganov,
Yosuke
Umehara,2
Vera A.
Voroshilova,
Arsen O.
Batagov,
Niels
Sandal,
Anita
Mortensen,
Leif
Schauser,
Noel
Ellis,
Igor A.
Tikhonovich, and
Jens
Stougaard*
All-Russia Research Institute for Agricultural Microbiology, St.
Petersburg, Pushkin 8, Podbelsky Chaussee, 3, 196608, Russia (A.Y.B.,
V.E.T., V.A.V., A.O.B., I.A.T.); Department of Molecular Biology,
Laboratory of Gene Expression, University of Aarhus, Gustav Wieds Vej
10, DK-8000, Aarhus C, Denmark (L.H.M., Y.U., N.S., A.M., L.S., J.S.);
and John Innes Centre, Norwich NR4 7UH, United Kingdom (N.E.)
Comparative phenotypic analysis of pea (Pisum
sativum) sym35 mutants and Lotus
japonicus nin mutants suggested a similar function for the
PsSym35 and LjNin genes in early stages
of root nodule formation. Both the pea and L.
japonicus mutants are non-nodulating but normal in their
arbuscular mycorrhizal association. Both are characterized by excessive
root hair curling in response to the bacterial microsymbiont, lack of
infection thread initiation, and absence of cortical cell divisions. To
investigate the molecular basis for the similarity, we cloned and
sequenced the PsNin gene, taking advantage of sequence
information from the previously cloned LjNin gene. An
RFLP analysis on recombinant inbred lines mapped PsNin
to the same chromosome arm as the PsSym35 locus and
direct evidence demonstrating that PsNin is the
PsSym35 gene was subsequently obtained by cosegregation
analysis and sequencing of three independent Pssym35
mutant alleles. L. japonicus and pea root nodules
develop through different organogenic pathways, so it was of interest to compare the expression of the two orthologous genes during nodule
formation. Overall, a similar developmental regulation of the
PsNin and LjNin genes was shown by the
transcriptional activation in root nodules of L.
japonicus and pea. In the indeterminate pea nodules,
PsNin is highly expressed in the meristematic cells of
zone I and in the cells of infection zone II, corroborating expression
of LjNin in determinate nodule primordia. At the protein level, seven domains, including the putative DNA binding/dimerization RWP-RK motif and the PB1 heterodimerization domain, are conserved between the LjNIN and PsNIN proteins.
1
This study was supported by the Russian
Foundation for Basic Research (grant nos. 01-04-49643 and
01-04-48580) and by the European INTAS program (grant no.
2322). A.Y.B.'s stay at Aarhus University was supported by the
European Molecular Biology Organization (short-term fellowship no. ASTF
9556) and N.S.'s stay at the John Innes was supported by The Danish
Agricultural and Veterinary Research Council.
2
Present address: Laboratory of Nitrogen Fixation,
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki
305-8602, Japan.
*
Corresponding author; e-mail stougaard{at}mbio.aau.dk; fax
45-86-201222.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
T. Vernie, S. Moreau, F. de Billy, J. Plet, J.-P. Combier, C. Rogers, G. Oldroyd, F. Frugier, A. Niebel, and P. Gamas
EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula
PLANT CELL,
October 1, 2008;
20(10):
2696 - 2713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhu, T. Chen, M. Zhu, Q. Fang, H. Kang, Z. Hong, and Z. Zhang
A Novel ARID DNA-Binding Protein Interacts with SymRK and Is Expressed during Early Nodule Development in Lotus japonicus
Plant Physiology,
September 1, 2008;
148(1):
337 - 347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Riano-Pachon, L. G. G. Correa, R. Trejos-Espinosa, and B. Mueller-Roeber
Green Transcription Factors: A Chlamydomonas Overview
Genetics,
May 1, 2008;
179(1):
31 - 39.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Smit, E. Limpens, R. Geurts, E. Fedorova, E. Dolgikh, C. Gough, and T. Bisseling
Medicago LYK3, an Entry Receptor in Rhizobial Nodulation Factor Signaling
Plant Physiology,
September 1, 2007;
145(1):
183 - 191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Sumimoto, S. Kamakura, and T. Ito
Structure and Function of the PB1 Domain, a Protein Interaction Module Conserved in Animals, Fungi, Amoebas, and Plants
Sci. Signal.,
August 28, 2007;
2007(401):
re6 - re6.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Lin and U. W. Goodenough
Gametogenesis in the Chlamydomonas reinhardtii minus Mating Type Is Controlled by Two Genes, MID and MTD1
Genetics,
June 1, 2007;
176(2):
913 - 925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Udvardi, K. Kakar, M. Wandrey, O. Montanari, J. Murray, A. Andriankaja, J.-Y. Zhang, V. Benedito, J. M.I. Hofer, F. Chueng, et al.
Legume Transcription Factors: Global Regulators of Plant Development and Response to the Environment
Plant Physiology,
June 1, 2007;
144(2):
538 - 549.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Marsh, A. Rakocevic, R. M. Mitra, L. Brocard, J. Sun, A. Eschstruth, S. R. Long, M. Schultze, P. Ratet, and G. E.D. Oldroyd
Medicago truncatula NIN Is Essential for Rhizobial-Independent Nodule Organogenesis Induced by Autoactive Calcium/Calmodulin-Dependent Protein Kinase
Plant Physiology,
May 1, 2007;
144(1):
324 - 335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhu, B. K. Riely, N. J. Burns, and J.-M. Ane
Tracing Nonlegume Orthologs of Legume Genes Required for Nodulation and Arbuscular Mycorrhizal Symbioses
Genetics,
April 1, 2006;
172(4):
2491 - 2499.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. O. Ovtsyna, E. A. Dolgikh, A. S. Kilanova, V. E. Tsyganov, A. Y. Borisov, I. A. Tikhonovich, and C. Staehelin
Nod Factors Induce Nod Factor Cleaving Enzymes in Pea Roots. Genetic and Pharmacological Approaches Indicate Different Activation Mechanisms
Plant Physiology,
October 1, 2005;
139(2):
1051 - 1064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Smit, J. Raedts, V. Portyanko, F. Debelle, C. Gough, T. Bisseling, and R. Geurts
NSP1 of the GRAS Protein Family Is Essential for Rhizobial Nod Factor-Induced Transcription
Science,
June 17, 2005;
308(5729):
1789 - 1791.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ooki, M. Banba, K. Yano, J. Maruya, S. Sato, S. Tabata, K. Saeki, M. Hayashi, M. Kawaguchi, K. Izui, et al.
Characterization of the Lotus japonicus Symbiotic Mutant lot1 That Shows a Reduced Nodule Number and Distorted Trichomes
Plant Physiology,
April 1, 2005;
137(4):
1261 - 1271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Szczyglowski and L. Amyot
Symbiosis, Inventiveness by Recruitment?
Plant Physiology,
March 1, 2003;
131(3):
935 - 940.
[Full Text]
[PDF]
|
|
|
|
|
