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Plant Physiol, March 2003, Vol. 131, pp. 1104-1123
Mapping the Proteome of Barrel Medic (Medicago
truncatula)1,[w]
Bonnie S.
Watson,
Victor S.
Asirvatham,
Liangjiang
Wang, and
Lloyd W.
Sumner*
Plant Biology Division, The Samuel Roberts Noble Foundation, P.O.
Box 2180, Ardmore, Oklahoma 73402
A survey of six organ-/tissue-specific proteomes of the
model legume barrel medic (Medicago truncatula) was
performed. Two-dimensional polyacrylamide gel electrophoresis reference
maps of protein extracts from leaves, stems, roots, flowers, seed pods,
and cell suspension cultures were obtained. Five hundred fifty-one
proteins were excised and 304 proteins identified using peptide mass
fingerprinting and matrix-assisted laser desorption ionization
time-of-flight mass spectrometry. Nanoscale high-performance liquid
chromatography coupled with tandem quadrupole time-of-flight mass
spectrometry was used to validate marginal matrix-assisted laser
desorption ionization time-of-flight mass spectrometry protein
identifications. This dataset represents one of the most comprehensive
plant proteome projects to date and provides a basis for future
proteome comparison of genetic mutants, biotically and abiotically
challenged plants, and/or environmentally challenged plants. Technical
details concerning peptide mass fingerprinting, database queries, and
protein identification success rates in the absence of a sequenced
genome are reported and discussed. A summary of the identified proteins
and their putative functions are presented. The tissue-specific
expression of proteins and the levels of identified proteins are
compared with their related transcript abundance as quantified through EST counting. It is estimated that approximately 50% of the proteins appear to be correlated with their corresponding mRNA levels.
1
This work was supported by the Samuel Roberts
Noble Foundation and by the National Science Foundation (Plant Genome
Research Project no. 0109732).
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail lwsumner{at}noble.org; fax
580-224-6692.
© 2003 American Society of Plant Biologists
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