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First published online February 13, 2003; 10.1104/pp.102.015974

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Plant Physiol, March 2003, Vol. 131, pp. 1137-1146

Pollination-, Development-, and Auxin-Specific Regulation of Gibberellin 3beta -Hydroxylase Gene Expression in Pea Fruit and Seeds1

Jocelyn A. Ozga,* Jody Yu, and Dennis M. Reinecke

Plant Physiology and Molecular Biology Research Group, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5

To understand further how pollination, seeds, auxin (4-chloroindole-3-acetic acid [4-Cl-IAA]), and gibberellins (GAs) regulate GA biosynthesis in pea (Pisum sativum) fruit, we studied expression of the gene PsGA3ox1 that codes for the enzyme that converts GA20 to biologically active GA1 using real-time reverse transcription-polymerase chain reaction analysis. PsGA3ox1 mRNA levels were minimally detectable in prepollinated pericarps and ovules (-2 d after anthesis [DAA]), increased dramatically after pollination (0 DAA), then decreased by 1 DAA. Seed PsGA3ox1 mRNA levels increased at 4 DAA and again 8 to 12 DAA, when seed development was rapid. Pericarp PsGA3ox1 mRNA levels peaked coincidentally with rapid pod diameter expansion (6-10 DAA) to accommodate the growing seeds. The effects of seeds and hormones on the expression of pericarp PsGA3ox1 were investigated over a 24-h treatment period. Pericarp PsGA3ox1 mRNA levels gradually increased from 2 to 3 DAA when seeds were present; however, when the seeds were removed, the pericarp transcript levels dramatically declined. When 2-DAA deseeded pericarps were treated with 4-Cl-IAA, PsGA3ox1 mRNA levels peaked 4 h after hormone treatment (270-fold increase), then decreased. PsGA3ox1 mRNA levels in deseeded pericarps treated with indole-3-acetic acid or GA3 were the same or lower than deseeded controls. These data show that PsGA3ox1 is expressed and developmentally regulated in pea pericarps and seeds. These data also show that pericarp PsGA3ox1 expression is hormonally regulated and suggest that the conversion of GA20 to GA1 occurs in the pericarp and is regulated by the presence of seeds and 4-Cl-IAA for fruit growth.

1 This research was supported by the National Sciences and Engineering Research Council of Canada (award no. OGP0138166 to J.A.O.).

* Corresponding author; e-mail jocelyn.ozga{at}ualberta.ca; fax 780-492-4265.

© 2003 American Society of Plant Biologists


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