First published online January 23, 2003; 10.1104/pp.010850
Plant Physiol, March 2003, Vol. 131, pp. 1228-1238
The Arabidopsis Mutant alh1 Illustrates a Cross
Talk between Ethylene and Auxin1
Filip
Vandenbussche,
Jan
Smalle,2
Jie
Le,
Nelson José Madeira
Saibo,
Annelies
De Paepe,
Laury
Chaerle,
Olaf
Tietz,
Raphael
Smets,
Lucas J.J.
Laarhoven,
Frans
J.M.
Harren,
Harry Van
Onckelen,
Klaus
Palme,
Jean-Pierre
Verbelen, and
Dominique
Van Der
Straeten*
Department of Plant Systems Biology, Flanders Interuniversity
Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium (F.V., J.S., N.J.M.S., A.D.P., L.C., D.V.D.S.);
Department of Biology, University of Antwerp, B-2610 Wilrijk, Belgium
(J.L., R.S., H.V.O., J.-P.V.); Institut für Biologie II,
Zellbiologie, University of Freiburg, 79104 Freiburg, Germany (O.T.,
K.P.); and Department of Molecular and Laser Physics, University of
Nijmegen, NL-6500 GL Nijmegen, The Netherlands (L.J.J.L.,
F.J.M.H.)
Ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid
(ACC) can stimulate hypocotyl elongation in light-grown Arabidopsis seedlings. A mutant, designated ACC-related long hypocotyl
1 (alh1), that displayed a long hypocotyl in the
light in the absence of the hormone was characterized. Etiolated
alh1 seedlings overproduced ethylene and had an
exaggerated apical hook and a thicker hypocotyl, although no difference
in hypocotyl length was observed when compared with wild type.
Alh1 plants were less sensitive to ethylene, as reflected by reduction of ACC-mediated inhibition of hypocotyl growth
in the dark and delay in flowering and leaf senescence. Alh1 also had an altered response to auxin, whereas
auxin levels in whole alh1 seedlings remained
unaffected. In contrast to wild type, alh1 seedlings
showed a limited hypocotyl elongation when treated with indole-3-acetic
acid. Alh1 roots had a faster response to gravity.
Furthermore, the hypocotyl elongation of alh1 and of
ACC-treated wild type was reverted by auxin transport inhibitors. In
addition, auxin up-regulated genes were ectopically expressed in
hypocotyls upon ACC treatment, suggesting that the ethylene response is
mediated by auxins. Together, these data indicate that
alh1 is altered in the cross talk between ethylene and
auxins, probably at the level of auxin transport.
1
This work was supported by the Fund for
Scientific Research (Flanders; grant nos. G.0281.98 and G.0345.02) and
by the European Union (grant for Access to Research Infrastructures
Action of the Improving Human Potential Program). L.C. is a
postdoctoral research assistant of the Fund for Scientific Research
(Flanders). A.D.P. is a Research Assistant of the Fund for Scientific
Research (Flanders).
2
Present address: College of Agricultural and Life
Sciences, University of Wisconsin, Madison, WI 53706.
*
Corresponding author; e-mail dostr{at}gengenp.rug.ac.be; fax
32-9-264-5349.
© 2003 American Society of Plant Biologists
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